Bibliographic Details
| Title: |
Variation of C-terminal domain governs RNA polymerase II genomic locations and alternative splicing in eukaryotic transcription |
| Authors: |
Qian Zhang, Wantae Kim, Svetlana B. Panina, Joshua E. Mayfield, Bede Portz, Y. Jessie Zhang |
| Source: |
Nature Communications, Vol 15, Iss 1, Pp 1-18 (2024) |
| Publisher Information: |
Nature Portfolio, 2024. |
| Publication Year: |
2024 |
| Collection: |
LCC:Science |
| Subject Terms: |
Science |
| More Details: |
Abstract The C-terminal domain of RPB1 (CTD) orchestrates transcription by recruiting regulators to RNA Pol II upon phosphorylation. With CTD driving condensate formation on gene loci, the molecular mechanism behind how CTD-mediated recruitment of transcriptional regulators influences condensates formation remains unclear. Our study unveils that phosphorylation reversibly dissolves phase separation induced by the unphosphorylated CTD. Phosphorylated CTD, upon specific association with transcription regulators, forms distinct condensates from unphosphorylated CTD. Functional studies demonstrate CTD variants with diverse condensation properties exhibit differences in promoter binding and mRNA co-processing in cells. Notably, varying CTD lengths influence the assembly of RNA processing machinery and alternative splicing outcomes, which in turn affects cellular growth, linking the evolution of CTD variation/length with the complexity of splicing from yeast to human. These findings provide compelling evidence for a model wherein post-translational modification enables the transition of functionally specialized condensates, highlighting a co-evolution link between CTD condensation and splicing. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2041-1723 |
| Relation: |
https://doaj.org/toc/2041-1723 |
| DOI: |
10.1038/s41467-024-52391-6 |
| Access URL: |
https://doaj.org/article/5617c07b006f4dc7b2e870a33e0063c5 |
| Accession Number: |
edsdoj.5617c07b006f4dc7b2e870a33e0063c5 |
| Database: |
Directory of Open Access Journals |
