Academic Journal
View in EDS

Nanoparticle-Based Lateral Flow Biosensors Integrated With Loop-Mediated Isothermal Amplification for the Rapid and Visual Diagnosis of Hepatitis B Virus in Clinical Application

Bibliographic Details
Title: Nanoparticle-Based Lateral Flow Biosensors Integrated With Loop-Mediated Isothermal Amplification for the Rapid and Visual Diagnosis of Hepatitis B Virus in Clinical Application
Authors: Xu Chen, Shoshi Wang, Yan Tan, Junfei Huang, Xingui Yang, Shijun Li
Source: Frontiers in Bioengineering and Biotechnology, Vol 9 (2021)
Publisher Information: Frontiers Media S.A., 2021.
Publication Year: 2021
Collection: LCC:Biotechnology
Subject Terms: hepatitis B virus, loop-mediated isothermal amplification, lateral flow biosensor, rapid of detection, limit of detection, Biotechnology, TP248.13-248.65
More Details: Hepatitis B virus (HBV) infection remains one of the major public health issues worldwide. Developing a rapid, sensitive, specific, easy-to-operate, and cost-saving approach for the diagnosis of HBV is essential for its therapy and prevention. Here, we first devised a novel approach, termed “loop-mediated isothermal amplification integrated with a nanoparticle-based lateral flow biosensor (LAMP-LFB),” for the detection of HBV in clinical application. The results indicated that a set of LAMP primers based on the S gene were valid for the establishment of HBV-LAMP-LFB. The optimal HBV-LAMP can be carried out at a constant temperature of 65°C for 40 min. The whole detection process, including HBV genomic DNA preparation (∼10 min), LAMP (40 min), and LFB reading (within 2 min), can be accomplished within 60 min. The limit of detection of the HBV-LAMP-LFB assay was 7.5 IU per test. The specificity of this assay was one hundred percent, and there was no cross-reactivity with other pathogens. Hence, these results indicated that the HBV-LAMP-LFB assay established in the current study is a sensitive, rapid, specific, visual, simple, and cost-saving method for the screening of HBV agents. More importantly, the HBV-LAMP-LFB has remarkable potential to develop a point-of-care testing in clinical application, especially in resource-scarce regions.
Document Type: article
File Description: electronic resource
Language: English
ISSN: 2296-4185
Relation: https://www.frontiersin.org/articles/10.3389/fbioe.2021.731415/full; https://doaj.org/toc/2296-4185
DOI: 10.3389/fbioe.2021.731415
Access URL: https://doaj.org/article/aee54ca2833c47bfb060490087cbdda1
Accession Number: edsdoj.54ca2833c47bfb060490087cbdda1
Database: Directory of Open Access Journals
FullText Text:
  Availability: 0
CustomLinks:
  – Url: https://resolver.ebsco.com/c/xy5jbn/result?sid=EBSCO:edsdoj&genre=article&issn=22964185&ISBN=&volume=9&issue=&date=20210901&spage=&pages=&title=Frontiers in Bioengineering and Biotechnology&atitle=Nanoparticle-Based%20Lateral%20Flow%20Biosensors%20Integrated%20With%20Loop-Mediated%20Isothermal%20Amplification%20for%20the%20Rapid%20and%20Visual%20Diagnosis%20of%20Hepatitis%20B%20Virus%20in%20Clinical%20Application&aulast=Xu%20Chen&id=DOI:10.3389/fbioe.2021.731415
    Name: Full Text Finder (for New FTF UI) (s8985755)
    Category: fullText
    Text: Find It @ SCU Libraries
    MouseOverText: Find It @ SCU Libraries
  – Url: https://doaj.org/article/aee54ca2833c47bfb060490087cbdda1
    Name: EDS - DOAJ (s8985755)
    Category: fullText
    Text: View record from DOAJ
    MouseOverText: View record from DOAJ
Header DbId: edsdoj
DbLabel: Directory of Open Access Journals
An: edsdoj.54ca2833c47bfb060490087cbdda1
RelevancyScore: 927
AccessLevel: 3
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 926.9267578125
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Nanoparticle-Based Lateral Flow Biosensors Integrated With Loop-Mediated Isothermal Amplification for the Rapid and Visual Diagnosis of Hepatitis B Virus in Clinical Application
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Xu+Chen%22">Xu Chen</searchLink><br /><searchLink fieldCode="AR" term="%22Shoshi+Wang%22">Shoshi Wang</searchLink><br /><searchLink fieldCode="AR" term="%22Yan+Tan%22">Yan Tan</searchLink><br /><searchLink fieldCode="AR" term="%22Junfei+Huang%22">Junfei Huang</searchLink><br /><searchLink fieldCode="AR" term="%22Xingui+Yang%22">Xingui Yang</searchLink><br /><searchLink fieldCode="AR" term="%22Shijun+Li%22">Shijun Li</searchLink>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: Frontiers in Bioengineering and Biotechnology, Vol 9 (2021)
– Name: Publisher
  Label: Publisher Information
  Group: PubInfo
  Data: Frontiers Media S.A., 2021.
– Name: DatePubCY
  Label: Publication Year
  Group: Date
  Data: 2021
– Name: Subset
  Label: Collection
  Group: HoldingsInfo
  Data: LCC:Biotechnology
– Name: Subject
  Label: Subject Terms
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22hepatitis+B+virus%22">hepatitis B virus</searchLink><br /><searchLink fieldCode="DE" term="%22loop-mediated+isothermal+amplification%22">loop-mediated isothermal amplification</searchLink><br /><searchLink fieldCode="DE" term="%22lateral+flow+biosensor%22">lateral flow biosensor</searchLink><br /><searchLink fieldCode="DE" term="%22rapid+of+detection%22">rapid of detection</searchLink><br /><searchLink fieldCode="DE" term="%22limit+of+detection%22">limit of detection</searchLink><br /><searchLink fieldCode="DE" term="%22Biotechnology%22">Biotechnology</searchLink><br /><searchLink fieldCode="DE" term="%22TP248%2E13-248%2E65%22">TP248.13-248.65</searchLink>
– Name: Abstract
  Label: Description
  Group: Ab
  Data: Hepatitis B virus (HBV) infection remains one of the major public health issues worldwide. Developing a rapid, sensitive, specific, easy-to-operate, and cost-saving approach for the diagnosis of HBV is essential for its therapy and prevention. Here, we first devised a novel approach, termed “loop-mediated isothermal amplification integrated with a nanoparticle-based lateral flow biosensor (LAMP-LFB),” for the detection of HBV in clinical application. The results indicated that a set of LAMP primers based on the S gene were valid for the establishment of HBV-LAMP-LFB. The optimal HBV-LAMP can be carried out at a constant temperature of 65°C for 40 min. The whole detection process, including HBV genomic DNA preparation (∼10 min), LAMP (40 min), and LFB reading (within 2 min), can be accomplished within 60 min. The limit of detection of the HBV-LAMP-LFB assay was 7.5 IU per test. The specificity of this assay was one hundred percent, and there was no cross-reactivity with other pathogens. Hence, these results indicated that the HBV-LAMP-LFB assay established in the current study is a sensitive, rapid, specific, visual, simple, and cost-saving method for the screening of HBV agents. More importantly, the HBV-LAMP-LFB has remarkable potential to develop a point-of-care testing in clinical application, especially in resource-scarce regions.
– Name: TypeDocument
  Label: Document Type
  Group: TypDoc
  Data: article
– Name: Format
  Label: File Description
  Group: SrcInfo
  Data: electronic resource
– Name: Language
  Label: Language
  Group: Lang
  Data: English
– Name: ISSN
  Label: ISSN
  Group: ISSN
  Data: 2296-4185
– Name: NoteTitleSource
  Label: Relation
  Group: SrcInfo
  Data: https://www.frontiersin.org/articles/10.3389/fbioe.2021.731415/full; https://doaj.org/toc/2296-4185
– Name: DOI
  Label: DOI
  Group: ID
  Data: 10.3389/fbioe.2021.731415
– Name: URL
  Label: Access URL
  Group: URL
  Data: <link linkTarget="URL" linkTerm="https://doaj.org/article/aee54ca2833c47bfb060490087cbdda1" linkWindow="_blank">https://doaj.org/article/aee54ca2833c47bfb060490087cbdda1</link>
– Name: AN
  Label: Accession Number
  Group: ID
  Data: edsdoj.54ca2833c47bfb060490087cbdda1
PLink https://login.libproxy.scu.edu/login?url=https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&scope=site&db=edsdoj&AN=edsdoj.54ca2833c47bfb060490087cbdda1
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.3389/fbioe.2021.731415
    Languages:
      – Text: English
    Subjects:
      – SubjectFull: hepatitis B virus
        Type: general
      – SubjectFull: loop-mediated isothermal amplification
        Type: general
      – SubjectFull: lateral flow biosensor
        Type: general
      – SubjectFull: rapid of detection
        Type: general
      – SubjectFull: limit of detection
        Type: general
      – SubjectFull: Biotechnology
        Type: general
      – SubjectFull: TP248.13-248.65
        Type: general
    Titles:
      – TitleFull: Nanoparticle-Based Lateral Flow Biosensors Integrated With Loop-Mediated Isothermal Amplification for the Rapid and Visual Diagnosis of Hepatitis B Virus in Clinical Application
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Xu Chen
      – PersonEntity:
          Name:
            NameFull: Shoshi Wang
      – PersonEntity:
          Name:
            NameFull: Yan Tan
      – PersonEntity:
          Name:
            NameFull: Junfei Huang
      – PersonEntity:
          Name:
            NameFull: Xingui Yang
      – PersonEntity:
          Name:
            NameFull: Shijun Li
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 09
              Type: published
              Y: 2021
          Identifiers:
            – Type: issn-print
              Value: 22964185
          Numbering:
            – Type: volume
              Value: 9
          Titles:
            – TitleFull: Frontiers in Bioengineering and Biotechnology
              Type: main
ResultId 1