Bibliographic Details
| Title: |
Nanoparticle-Based Lateral Flow Biosensors Integrated With Loop-Mediated Isothermal Amplification for the Rapid and Visual Diagnosis of Hepatitis B Virus in Clinical Application |
| Authors: |
Xu Chen, Shoshi Wang, Yan Tan, Junfei Huang, Xingui Yang, Shijun Li |
| Source: |
Frontiers in Bioengineering and Biotechnology, Vol 9 (2021) |
| Publisher Information: |
Frontiers Media S.A., 2021. |
| Publication Year: |
2021 |
| Collection: |
LCC:Biotechnology |
| Subject Terms: |
hepatitis B virus, loop-mediated isothermal amplification, lateral flow biosensor, rapid of detection, limit of detection, Biotechnology, TP248.13-248.65 |
| More Details: |
Hepatitis B virus (HBV) infection remains one of the major public health issues worldwide. Developing a rapid, sensitive, specific, easy-to-operate, and cost-saving approach for the diagnosis of HBV is essential for its therapy and prevention. Here, we first devised a novel approach, termed “loop-mediated isothermal amplification integrated with a nanoparticle-based lateral flow biosensor (LAMP-LFB),” for the detection of HBV in clinical application. The results indicated that a set of LAMP primers based on the S gene were valid for the establishment of HBV-LAMP-LFB. The optimal HBV-LAMP can be carried out at a constant temperature of 65°C for 40 min. The whole detection process, including HBV genomic DNA preparation (∼10 min), LAMP (40 min), and LFB reading (within 2 min), can be accomplished within 60 min. The limit of detection of the HBV-LAMP-LFB assay was 7.5 IU per test. The specificity of this assay was one hundred percent, and there was no cross-reactivity with other pathogens. Hence, these results indicated that the HBV-LAMP-LFB assay established in the current study is a sensitive, rapid, specific, visual, simple, and cost-saving method for the screening of HBV agents. More importantly, the HBV-LAMP-LFB has remarkable potential to develop a point-of-care testing in clinical application, especially in resource-scarce regions. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2296-4185 |
| Relation: |
https://www.frontiersin.org/articles/10.3389/fbioe.2021.731415/full; https://doaj.org/toc/2296-4185 |
| DOI: |
10.3389/fbioe.2021.731415 |
| Access URL: |
https://doaj.org/article/aee54ca2833c47bfb060490087cbdda1 |
| Accession Number: |
edsdoj.54ca2833c47bfb060490087cbdda1 |
| Database: |
Directory of Open Access Journals |
