Identification of Differentially Expressed Genes by cDNA-AFLP in Magnaporthe oryzae
| Title: | Identification of Differentially Expressed Genes by cDNA-AFLP in Magnaporthe oryzae |
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| Authors: | Myoung-Hwan Chi, Sook-Young Park |
| Source: | Research in Plant Disease, Vol 25, Iss 4, Pp 205-212 (2019) |
| Publisher Information: | Hanrimwon Publishing Company, 2019. |
| Publication Year: | 2019 |
| Collection: | LCC:Agriculture (General) |
| Subject Terms: | appressorium formation, cdna-aflp, gene expression, magnaporthe oryzae, qrt-pcr, Agriculture (General), S1-972 |
| More Details: | Analysis of differentially expressed genes has assisted discovery of gene sets involved in particular biological processes. The purpose of this study was to identify genes involved in appressorium formation in the rice blast fungus Magnaporthe oryzae via analysis of cDNA–amplified fragment length polymorphisms. Amplification of appressorial and vegetative mycelial cDNAs using 28 primer combinations generated over 200 differentially expressed transcript-derived fragments (TDFs). TDFs were excised from gels, re-amplified by PCR, cloned, and sequenced. Forty-four of 52 clones analyzed corresponded to 42 genes. Quantitative real-time PCR showed that expression of 23 genes was up-regulated during appressorium formation, one of which was the MCK1 gene that had been shown to be involved in appressorium formation. This study will be providing valuable resources for identifying the genes such as pathogenicity-related genes in M. oryzae. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English Korean |
| ISSN: | 1598-2262 |
| Relation: | http://www.online-rpd.org/journal/view.html?doi=10.5423/RPD.2019.25.4.205; https://doaj.org/toc/1598-2262 |
| DOI: | 10.5423/RPD.2019.25.4.205 |
| Access URL: | https://doaj.org/article/52ba1e92872d4fb797dce2bc7c3cb9e2 |
| Accession Number: | edsdoj.52ba1e92872d4fb797dce2bc7c3cb9e2 |
| Database: | Directory of Open Access Journals |
| ISSN: | 15982262 |
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| DOI: | 10.5423/RPD.2019.25.4.205 |
| Published in: | Research in Plant Disease |
| Language: | English Korean |