Bibliographic Details
| Title: |
Extraction and purification of ustiloxin A from rice false smut balls by a combination of macroporous resin and high-speed countercurrent chromatography |
| Authors: |
Gang Wang, Dan He, Fengchun Zhao, Junqiang Hu, Yin-Won Lee, Jianrong Shi, Jianhong Xu |
| Source: |
Food Production, Processing and Nutrition, Vol 2, Iss 1, Pp 1-13 (2020) |
| Publisher Information: |
BMC, 2020. |
| Publication Year: |
2020 |
| Collection: |
LCC:Nutrition. Foods and food supply
LCC:Food processing and manufacture |
| Subject Terms: |
Ustiloxin A, Rice false smut, Preparative purification, Macroporous resin, High-speed counter-current chromatography, Phytotoxicity assay, Nutrition. Foods and food supply, TX341-641, Food processing and manufacture, TP368-456 |
| More Details: |
Abstract Rice false smut is an emerging plant disease worldwide. Ustiloxin A (UstA) is the major mycotoxin found in rice false smut balls, which are fungal colonies in rice florets. In this study, a new method consisting of macroporous resin column chromatography and high-speed countercurrent chromatography (HSCCC) was developed for UstA separation. UstA was extracted by a 3.81% HCOOH solution and adsorbed by XAD-4 resin. UstA was then eluted by a 40% methanol solution supplemented with 0.1% trifluoroacetic acid (TFA). Further purification was achieved by HSCCC using a two-phase solvent system consisting of n-butanol/TFA/H2O (1/0.05/1, v/v/v). Under the optimized conditions, 225 mg of UstA was obtained with a purity of 97.39% in a single run, with a final recovery of 65.2%. An inhibitory effect on seed germination of wheat and maize caused by UstA was observed in a preliminary phytotoxicity assay. Graphical abstract |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2661-8974 |
| Relation: |
https://doaj.org/toc/2661-8974 |
| DOI: |
10.1186/s43014-020-00043-9 |
| Access URL: |
https://doaj.org/article/52b70ec450484e05bfda13f50fdf2fae |
| Accession Number: |
edsdoj.52b70ec450484e05bfda13f50fdf2fae |
| Database: |
Directory of Open Access Journals |
