Bibliographic Details
| Title: |
Soil Actinobacteria Exhibit Metabolic Capabilities for Degrading the Toxic and Persistent Herbicide Metribuzin |
| Authors: |
Hadjer Rebai, Essam Nageh Sholkamy, Mohamed A. A. Abdelhamid, Pratheesh Prakasam Thanka, Ashraf Aly Hassan, Seung Pil Pack, Mi-Ran Ki, Allaoueddine Boudemagh |
| Source: |
Toxics, Vol 12, Iss 10, p 709 (2024) |
| Publisher Information: |
MDPI AG, 2024. |
| Publication Year: |
2024 |
| Collection: |
LCC:Chemical technology |
| Subject Terms: |
triazine, metribuzin, biodegradation, microorganisms, Streptomyces, agricultural soil, Chemical technology, TP1-1185 |
| More Details: |
Metribuzin, a widely used triazine herbicide, persists in agricultural soils and poses significant environmental pollution threats globally. The aim of this study was to investigate the biodegradation of metribuzin by actinobacterial strains in vitro at different environmental conditions. From an initial screen of 12 actinobacterial strains, four bacteria exhibited robust growth in the presence of the metribuzin as the sole carbon source at 50 mg/L concentration. The optimization of metribuzin biodegradation under different conditions (pH, temperature and inoculum size) using a spectrophotometric method revealed that maximum degradation of metribuzin occurred at a pH of 7.2, a temperature 30 °C, and at an inoculum volume of 4%. Subsequent GC-MS validation confirmed the remarkable biodegradation capabilities of the actinobacterial isolates, where the strain C1 showed the highest rate of metribuzin degradation of 83.12%. Detailed phylogenetic identified the active strains as Streptomyces toxytricini (CH), Streptomyces stelliscabiei (B2), and two Streptomyces heliomycini (C1, C3). Structural analysis by ATR-FTIR spectroscopy confirmed the extensive biotransformation of the herbicide molecule. Our findings highlight the immense untapped potential of soil actinobacteria, particularly the Streptomyces heliomycini C1 strain, as versatile bioremediation agents for removing persistent agrochemical pollutants. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2305-6304 |
| Relation: |
https://www.mdpi.com/2305-6304/12/10/709; https://doaj.org/toc/2305-6304 |
| DOI: |
10.3390/toxics12100709 |
| Access URL: |
https://doaj.org/article/52512efbda2746bfbe13b9d7d9e9e9be |
| Accession Number: |
edsdoj.52512efbda2746bfbe13b9d7d9e9e9be |
| Database: |
Directory of Open Access Journals |
