Secretory carrier-associated membrane protein 5 regulates cell-surface targeting of T-type calcium channels
| Title: | Secretory carrier-associated membrane protein 5 regulates cell-surface targeting of T-type calcium channels |
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| Authors: | Emilio R. Mustafá, Konstantin Weiß, Norbert Weiss |
| Source: | Channels, Vol 17, Iss 1 (2023) |
| Publisher Information: | Taylor & Francis Group, 2023. |
| Publication Year: | 2023 |
| Collection: | LCC:Therapeutics. Pharmacology LCC:Physiology |
| Subject Terms: | Ion channels, Calcium channels, T-type channels, Secretory carrier-associated membrane protein 5, SCAMP5, Channelopathy, Therapeutics. Pharmacology, RM1-950, Physiology, QP1-981 |
| More Details: | ABSTRACTMissense mutations in the human secretary carrier-associated membrane protein 5 (SCAMP5) cause a variety of neurological disorders including neurodevelopmental delay, epilepsy, and Parkinson’s disease. We recently documented the importance of SCAMP2 in the regulation of T-type calcium channel expression in the plasma membrane. Here, we show that similar to SCAMP2, the co-expression of SCAMP5 in tsA-201 cells expressing recombinant Cav3.1, Cav3.2, and Cav3.3 channels nearly abolished whole-cell T-type currents. Recording of intramembrane charge movements revealed that SCAMP5-induced inhibition of T-type currents is primarily caused by the reduced expression of functional channels in the plasma membrane. Moreover, we show that SCAMP5-mediated downregulation of Cav3.2 channels is essentially preserved with disease-causing SCAMP5 R91W and G180W mutations. Hence, this study extends our previous findings with SCAMP2 and indicates that SCAMP5 also contributes to repressing the expression of T-type channels in the plasma membrane. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 19336950 1933-6969 1933-6950 |
| Relation: | https://doaj.org/toc/1933-6950; https://doaj.org/toc/1933-6969 |
| DOI: | 10.1080/19336950.2023.2230776 |
| Access URL: | https://doaj.org/article/5237809d9ba44036b7d3575a220a22e4 |
| Accession Number: | edsdoj.5237809d9ba44036b7d3575a220a22e4 |
| Database: | Directory of Open Access Journals |
| ISSN: | 19336950 19336969 |
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| DOI: | 10.1080/19336950.2023.2230776 |
| Published in: | Channels |
| Language: | English |