Bibliographic Details
| Title: |
Mutations in the Two-Component GluS-GluR Regulatory System Confer Resistance to β-Lactam Antibiotics in Burkholderia glumae |
| Authors: |
Joan Marunga, Eunhye Goo, Yongsung Kang, Ingyu Hwang |
| Source: |
Frontiers in Microbiology, Vol 12 (2021) |
| Publisher Information: |
Frontiers Media S.A., 2021. |
| Publication Year: |
2021 |
| Collection: |
LCC:Microbiology |
| Subject Terms: |
β-lactam resistance, β-lactamase, Burkholderia glumae, penicillin-binding protein, rice panicle blight, two-component systems, Microbiology, QR1-502 |
| More Details: |
Bacteria have specific signaling systems to overcome selective pressure, such as exposure to antibiotics. The two-component system (TCS) plays an important role in the development of antibiotic resistance. Using the rice pathogen Burkholderia glumae BGR1 as a model organism, we showed that the GluS (BGLU_1G13350) – GluR (BGLU_1G13360) TCS, consisting of a sensor kinase and response regulator, respectively, contributes to β-lactam resistance through a distinct mechanism. Inactivation of gluS or gluR conferred resistance to β-lactam antibiotics in B. glumae, whereas wild-type (WT) B. glumae was susceptible to these antibiotics. In gluS and gluR mutants, the expression of genes encoding metallo-β-lactamases (MBLs) and penicillin-binding proteins (PBPs) was significantly higher than in the WT. GluR-His bound to the putative promoter regions of annotated genes encoding MBL (BGLU_1G21360) and PBPs (BGLU_1G13280 and BGLU_1G04560), functioning as a repressor. These results demonstrate that the potential to attain β-lactam resistance may be genetically concealed in the TCS, in contrast to the widely accepted view of the role of TCS in antibiotic resistance. Our findings provide a new perspective on antibiotic resistance mechanisms, and suggest a different therapeutic approach for successful control of bacterial pathogens. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1664-302X |
| Relation: |
https://www.frontiersin.org/articles/10.3389/fmicb.2021.721444/full; https://doaj.org/toc/1664-302X |
| DOI: |
10.3389/fmicb.2021.721444 |
| Access URL: |
https://doaj.org/article/ed5192c1a6de419fb9932f6cf9ca119f |
| Accession Number: |
edsdoj.5192c1a6de419fb9932f6cf9ca119f |
| Database: |
Directory of Open Access Journals |
