Gene Expression Profiles in Primary Sjögren's Syndrome With and Without Systemic Manifestations

Bibliographic Details
Title: Gene Expression Profiles in Primary Sjögren's Syndrome With and Without Systemic Manifestations
Authors: Claudio Vitali, Marzia Dolcino, Nicoletta Del Papa, Antonina Minniti, Francesca Pignataro, Wanda Maglione, Claudio Lunardi, Antonio Puccetti
Source: ACR Open Rheumatology, Vol 1, Iss 10, Pp 603-613 (2019)
Publisher Information: Wiley, 2019.
Publication Year: 2019
Collection: LCC:Diseases of the musculoskeletal system
Subject Terms: Diseases of the musculoskeletal system, RC925-935
More Details: Objective To investigate the gene expression profile in patients with Sjögren's syndrome that is characterized by different clinical phenotypes. Methods RNA from peripheral blood mononuclear cells was purified in 8 patients with glandular features (GFs) and widespread pain (WP) and 11 with extraglandular manifestations (EGMs) and then was analyzed by hybridization on a human gene chip exploring more than 40,000 human genes. Differentially expressed genes (DEGs) in the two subgroups (ie, those with false discovery rate–corrected P values ≤ 0.01) with respect to 20 healthy controls have been submitted to functional classification using a Gene Ontology database and were mapped to define the networks of protein to protein interactions (PPIs). Results The enriched pathway analyses of DEGs and of the highly interconnected modules identified in the PPI networks showed that the pathological processes characterizing the two subgroups were substantially different. The predominant pathways in patients with EGMs are related to T‐ and B‐cell activation, Toll‐like receptor, interferon signaling, and apoptosis. Conversely, pathological processes related to pain transmission and modulation are preferentially operative in patients with GFs and WP. These data suggest that a neuroinflammatory pathway driven by cytokines and chemokines may play a central role in triggering WP features in this phenotype of patients. Conclusion The present study supports the hypothesis that different biological pathways are operative in patients with primary Sjögren's syndrome with different clinical phenotypes. A better knowledge of these specific processes might help in tailoring more effective target therapies.
Document Type: article
File Description: electronic resource
Language: English
ISSN: 2578-5745
Relation: https://doaj.org/toc/2578-5745
DOI: 10.1002/acr2.11082
Access URL: https://doaj.org/article/e5127703a05c487e847f26007b7364ab
Accession Number: edsdoj.5127703a05c487e847f26007b7364ab
Database: Directory of Open Access Journals
More Details
ISSN:25785745
DOI:10.1002/acr2.11082
Published in:ACR Open Rheumatology
Language:English