Bibliographic Details
| Title: |
PIRCh-seq: functional classification of non-coding RNAs associated with distinct histone modifications |
| Authors: |
Jingwen Fang, Qing Ma, Ci Chu, Beibei Huang, Lingjie Li, Pengfei Cai, Pedro J. Batista, Karen Erisse Martin Tolentino, Jin Xu, Rui Li, Pengcheng Du, Kun Qu, Howard Y. Chang |
| Source: |
Genome Biology, Vol 20, Iss 1, Pp 1-21 (2019) |
| Publisher Information: |
BMC, 2019. |
| Publication Year: |
2019 |
| Collection: |
LCC:Biology (General)
LCC:Genetics |
| Subject Terms: |
Biology (General), QH301-705.5, Genetics, QH426-470 |
| More Details: |
Abstract We develop PIRCh-seq, a method which enables a comprehensive survey of chromatin-associated RNAs in a histone modification-specific manner. We identify hundreds of chromatin-associated RNAs in several cell types with substantially less contamination by nascent transcripts. Non-coding RNAs are found enriched on chromatin and are classified into functional groups based on the patterns of their association with specific histone modifications. We find single-stranded RNA bases are more chromatin-associated, and we discover hundreds of allele-specific RNA-chromatin interactions. These results provide a unique resource to globally study the functions of chromatin-associated lncRNAs and elucidate the basic mechanisms of chromatin-RNA interactions. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1474-760X |
| Relation: |
https://doaj.org/toc/1474-760X |
| DOI: |
10.1186/s13059-019-1880-3 |
| Access URL: |
https://doaj.org/article/5122f9dc0f75472ca820e5eee9f8092a |
| Accession Number: |
edsdoj.5122f9dc0f75472ca820e5eee9f8092a |
| Database: |
Directory of Open Access Journals |
