Bibliographic Details
| Title: |
Evaluation of Human Platelet Lysate as an Alternative to Fetal Bovine Serum for Potential Clinical Applications of Stem Cells from Human Exfoliated Deciduous Teeth |
| Authors: |
Ji-Young Yoon, Huong Thu Vu, Jun Hee Lee, Ji-Sun Shin, Hae-Won Kim, Hae-Hyoung Lee, Jong-Bin Kim, Jung-Hwan Lee |
| Source: |
Cells, Vol 13, Iss 10, p 847 (2024) |
| Publisher Information: |
MDPI AG, 2024. |
| Publication Year: |
2024 |
| Collection: |
LCC:Cytology |
| Subject Terms: |
stem cells derived from human exfoliated deciduous teeth, human dental pulp stem cells, human platelet lysate, SHED, DPSC, hPL, Cytology, QH573-671 |
| More Details: |
In recent years, there has been a surge in demand for and research focus on cell therapy, driven by the tissue-regenerative and disease-treating potentials of stem cells. Among the candidates, dental pulp stem cells (DPSCs) or human exfoliated deciduous teeth (SHED) have garnered significant attention due to their easy accessibility (non-invasive), multi-lineage differentiation capability (especially neurogenesis), and low immunogenicity. Utilizing these stem cells for clinical purposes requires careful culture techniques such as excluding animal-derived supplements. Human platelet lysate (hPL) has emerged as a safer alternative to fetal bovine serum (FBS) for cell culture. In our study, we assessed the impact of hPL as a growth factor supplement for culture medium, also conducting a characterization of SHED cultured in hPL-supplemented medium (hPL-SHED). The results showed that hPL has effects in enhancing cell proliferation and migration and increasing cell survivability in oxidative stress conditions induced by H2O2. The morphology of hPL-SHED exhibited reduced size and elongation, with a differentiation capacity comparable to or even exceeding that of SHED cultured in a medium supplemented with fetal bovine serum (FBS-SHED). Moreover, no evidence of chromosome abnormalities or tumor formation was detected. In conclusion, hPL-SHED emerges as a promising candidate for cell therapy, exhibiting considerable potential for clinical investigation. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2073-4409 |
| Relation: |
https://www.mdpi.com/2073-4409/13/10/847; https://doaj.org/toc/2073-4409 |
| DOI: |
10.3390/cells13100847 |
| Access URL: |
https://doaj.org/article/50be2cce333e445b947bc037a17a73c5 |
| Accession Number: |
edsdoj.50be2cce333e445b947bc037a17a73c5 |
| Database: |
Directory of Open Access Journals |
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