Probucol Protects Endothelial Progenitor Cells Against Oxidized Low-Density Lipoprotein via Suppression of Reactive Oxygen Species Formation In Vivo
| Title: | Probucol Protects Endothelial Progenitor Cells Against Oxidized Low-Density Lipoprotein via Suppression of Reactive Oxygen Species Formation In Vivo |
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| Authors: | Qingbin Zhang, Liming Chen, Zhihua Si, Haoran Bu, Chandrakala A. Narasimhulu, Xueling Song, Ming-Yu Cui, Hang Liu, Tiewei Lu, Guanglong He, Sampath Parthasarathy, Lianqun Cui, Zhenguo Liu, Yuqi Cui |
| Source: | Cellular Physiology and Biochemistry, Vol 39, Iss 1, Pp 89-101 (2016) |
| Publisher Information: | Cell Physiol Biochem Press GmbH & Co KG, 2016. |
| Publication Year: | 2016 |
| Collection: | LCC:Physiology LCC:Biochemistry |
| Subject Terms: | Probucol, Endothelial progenitor cells, Oxidized low-density lipoprotein, Reactive oxygen species, Physiology, QP1-981, Biochemistry, QD415-436 |
| More Details: | Background/Aims: Oxidized low-density lipoprotein (ox-LDL) is a major component of hyperlipidemia and contributes to atherosclerosis. Endothelial progenitor cells (EPCs) play an important role in preventing atherosclerosis and notably decreased in hyperlipidemia. Ox-LDL and ox-LDL-related reactive oxygen species (ROS) have deleterious effects on EPCs. Probucol as an antioxidant and anti-inflammatory drug reduces ROS production. The present study was to determine if probucol could protect EPCs from ox-LDL in vivo and to investigate the potential mechanisms. Methods: ox-LDL was injected into male C57BL/6 mice for 3 days with or without probucol treatment with PBS as control. Bone marrow (BM) fluid, serum, circulating mononuclear cells (MNCs) and EPCs were collected for analysis. Results: the increased extracellular ROS in BM, serum and blood intracellular ROS production in the mice with ox-LDL treatment in association with a significant reduction of circulating MNCs and EPCs were restored with Probucol treatment. A significant increase in the serum ox-LDL and C-reactive protein and decrease in superoxide dismutase and circulating MNCs and EPCs were observed in hyperlipidemic patients that were effectively reversed with probucol treatment. Conclusion: these data suggested that probucol could protect EPCs from ox-LDL through inhibition of ROS production in vivo. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 1015-8987 1421-9778 |
| Relation: | http://www.karger.com/Article/FullText/445608; https://doaj.org/toc/1015-8987; https://doaj.org/toc/1421-9778 |
| DOI: | 10.1159/000445608 |
| Access URL: | https://doaj.org/article/50296512ee2c41dea4df6f6c6df25b79 |
| Accession Number: | edsdoj.50296512ee2c41dea4df6f6c6df25b79 |
| Database: | Directory of Open Access Journals |
| ISSN: | 10158987 14219778 |
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| DOI: | 10.1159/000445608 |
| Published in: | Cellular Physiology and Biochemistry |
| Language: | English |