Quercetin synergistically potentiates the anti-metastatic effect of 5-fluorouracil on the MDA-MB-231 breast cancer cell line
| Title: | Quercetin synergistically potentiates the anti-metastatic effect of 5-fluorouracil on the MDA-MB-231 breast cancer cell line |
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| Authors: | Mohammadreza Roshanazadeh, hossein babaahmadi rezaei, Mojtaba Rashidi |
| Source: | Iranian Journal of Basic Medical Sciences, Vol 24, Iss 7, Pp 928-934 (2021) |
| Publisher Information: | Mashhad University of Medical Sciences, 2021. |
| Publication Year: | 2021 |
| Collection: | LCC:Medicine |
| Subject Terms: | 5-fluorouracil, anti-metastatic effect, breast cancer, quercetin, synergism, Medicine |
| More Details: | Objective(s): Breast cancer (BC) cells’ ability to metastasize to other tissues increases mortality. The Matrix metalloproteinases 2 and 9 (MMP-2 and MMP-9) facilitate cancer cell migration. 5-fluorouracil is a frequently applied chemotherapeutic agent in cancer treatment with destructive side effects on normal tissues. Hence, researchers have focused on finding a way to reduce the dose of chemotherapeutic drugs. Quercetin, a natural polyphenolic compound, has inhibitory effects on proliferation and migration of tumor cells. This study evaluated the effect of the combination of Quercetin and 5-fluorouracil on migration of the MDA-MB-231 breast cancer cell line. Materials and Methods: The effect of Quercetin, 5-fluorouracil , and their combination on MDA-MB-231 breast cancer cell proliferation was investigated through MTT assay. Inhibition of tumor cell migration was examined by wound healing assay. Finally, the effect of treatments on gene expression of MMP-2 and MMP-9 was evaluated by quantitative real-time PCR. Results: The IC50 values for Quercetin and 5-fluorouracil after 48 hr treatment were 295 μM and 525 μM, respectively. The combination index (CI) for Quercetin and 5-fluorouracil was |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 2008-3866 2008-3874 |
| Relation: | https://ijbms.mums.ac.ir/article_18252_3005681d45eb5f619f6a5e688de77128.pdf; https://doaj.org/toc/2008-3866; https://doaj.org/toc/2008-3874 |
| DOI: | 10.22038/ijbms.2021.56559.12629 |
| Access URL: | https://doaj.org/article/4fe8e6ef15654e39b3f83ca76d94a38f |
| Accession Number: | edsdoj.4fe8e6ef15654e39b3f83ca76d94a38f |
| Database: | Directory of Open Access Journals |
| ISSN: | 20083866 20083874 |
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| DOI: | 10.22038/ijbms.2021.56559.12629 |
| Published in: | Iranian Journal of Basic Medical Sciences |
| Language: | English |