Bibliographic Details
| Title: |
In vitro and in vivo models define a molecular signature reference for human embryonic notochordal cells |
| Authors: |
Julie Warin, Nicolas Vedrenne, Vivian Tam, Mengxia Zhu, Danqing Yin, Xinyi Lin, Bluwen Guidoux-D’halluin, Antoine Humeau, Luce Roseiro, Lily Paillat, Claire Chédeville, Caroline Chariau, Frank Riemers, Markus Templin, Jérôme Guicheux, Marianna A. Tryfonidou, Joshua W.K. Ho, Laurent David, Danny Chan, Anne Camus |
| Source: |
iScience, Vol 27, Iss 2, Pp 109018- (2024) |
| Publisher Information: |
Elsevier, 2024. |
| Publication Year: |
2024 |
| Collection: |
LCC:Science |
| Subject Terms: |
Biological sciences, Biology of human development, Developmental biology, Natural sciences, Omics, Transcriptomics, Science |
| More Details: |
Summary: Understanding the emergence of human notochordal cells (NC) is essential for the development of regenerative approaches. We present a comprehensive investigation into the specification and generation of bona fide NC using a straightforward pluripotent stem cell (PSC)-based system benchmarked with human fetal notochord. By integrating in vitro and in vivo transcriptomic data at single-cell resolution, we establish an extended molecular signature and overcome the limitations associated with studying human notochordal lineage at early developmental stages. We show that TGF-β inhibition enhances the yield and homogeneity of notochordal lineage commitment in vitro. Furthermore, this study characterizes regulators of cell-fate decision and matrisome enriched in the notochordal niche. Importantly, we identify specific cell-surface markers opening avenues for differentiation refinement, NC purification, and functional studies. Altogether, this study provides a human notochord transcriptomic reference that will serve as a resource for notochord identification in human systems, diseased-tissues modeling, and facilitating future biomedical research. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2589-0042 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S2589004224002396; https://doaj.org/toc/2589-0042 |
| DOI: |
10.1016/j.isci.2024.109018 |
| Access URL: |
https://doaj.org/article/4fbbade5e5f24b0385edeacb652fc23d |
| Accession Number: |
edsdoj.4fbbade5e5f24b0385edeacb652fc23d |
| Database: |
Directory of Open Access Journals |
