Bibliographic Details
| Title: |
Protocol for the generation of pancreatic and hepatic progenitors from human pluripotent stem cells for gene regulatory assays |
| Authors: |
Lay Shuen Tan, Resilind Su Ern Chew, Natasha Hui Jin Ng, Adrian Kee Keong Teo |
| Source: |
STAR Protocols, Vol 2, Iss 2, Pp 100471- (2021) |
| Publisher Information: |
Elsevier, 2021. |
| Publication Year: |
2021 |
| Collection: |
LCC:Science (General) |
| Subject Terms: |
Cell culture, Cell-based Assays, Molecular Biology, Gene Expression, Stem Cells, Cell Differentiation, Science (General), Q1-390 |
| More Details: |
Summary: This protocol describes the detailed procedures for utilizing human pluripotent stem cells (hPSCs) for pancreatic progenitor and hepatic differentiation, followed by the application of hPSC-derived cells in a luciferase reporter-based assay to study gene regulation. The generated hPSC-derived cells have been shown to achieve morphologies and gene expression profiles specific to their differentiated cell types, and subsequent luciferase assay has been shown to effectively elucidate the role of disease-relevant gene variants. Therefore, this protocol provides a valuable approach for pancreatic and liver disease modeling.For complete details on the use and execution of this protocol, please refer to Ng et al. (2019). |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2666-1667 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S2666166721001787; https://doaj.org/toc/2666-1667 |
| DOI: |
10.1016/j.xpro.2021.100471 |
| Access URL: |
https://doaj.org/article/4f2a3b20b9ef429c80c481f160077319 |
| Accession Number: |
edsdoj.4f2a3b20b9ef429c80c481f160077319 |
| Database: |
Directory of Open Access Journals |
