Academic Journal
Evaluation of an HIV recent infection testing algorithm with serological assays among men who have sex with men in Mexico
| Title: | Evaluation of an HIV recent infection testing algorithm with serological assays among men who have sex with men in Mexico |
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| Authors: | Eli F. Karatzas-Delgado, Verónica Ruiz-González, Santa García-Cisneros, María L. Olamendi-Portugal, Antonia Herrera-Ortiz, Hugo López-Gatell, Andrea González-Rodríguez, Miguel A. Sánchez-Alemán |
| Source: | Journal of Infection and Public Health, Vol 13, Iss 4, Pp 509-513 (2020) |
| Publisher Information: | Elsevier, 2020. |
| Publication Year: | 2020 |
| Collection: | LCC:Infectious and parasitic diseases LCC:Public aspects of medicine |
| Subject Terms: | Infectious and parasitic diseases, RC109-216, Public aspects of medicine, RA1-1270 |
| More Details: | Background: Human immunodeficiency virus (HIV) incidence should be calculated in cross-sectional studies using recent infection testing algorithms (RITA) that consider clinical variables and serological test results such as enzyme-linked immunosorbent assay (ELISA) and dried blood spot (DBS) analysis. Methods: The correlation between serum samples and DBS was evaluated using two commercial ELISA kits: SediaTM BED HIV-1 Incidence EIA (BED-Sedia) and Maxim HIV-1 Limiting Antigen Avidity (LAg-Avidity). Eight different RITAs were developed; all of them included serological assays. A combination of the variables viral load, antiretroviral therapy (ART) and CD4 count was used to build the RITAs. The sensitivity, specificity, Youden index, predictive positive value, predictive negative value, false recent rate (FRR) and false long-term rate were evaluated. Results: The correlations between serum samples and DBS were 0.990 and 0.867 for BED-Sedia and LAg-avidity, respectively. Using only serological assays, the Youden index was higher for LAg-avidity than BED-Sedia (82.1–83.0% versus 69.2–69.6%). The best RITA was ART-serology, which showed a Youden index of 91.2–93.9% and FRR of 1.8–2.2%. Conclusions: Using DBS samples to determine HIV incidence is a good tool for epidemiological surveillance. The RITA that included ART and serological tests (BED-Sedia or LAg-avidity) showed the highest sensitivity and specificity and a low FRR. Keywords: HIV serodiagnosis, Incidence, Dried blood spot testing, Serologic tests |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 1876-0341 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S1876034119303399; https://doaj.org/toc/1876-0341 |
| DOI: | 10.1016/j.jiph.2019.11.002 |
| Access URL: | https://doaj.org/article/4ee0821eb1374357a793ad6428a6a18e |
| Accession Number: | edsdoj.4ee0821eb1374357a793ad6428a6a18e |
| Database: | Directory of Open Access Journals |
| ISSN: | 18760341 |
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| DOI: | 10.1016/j.jiph.2019.11.002 |
| Published in: | Journal of Infection and Public Health |
| Language: | English |