Bibliographic Details
| Title: |
Astrocytes profiling in acute hepatic encephalopathy: Possible enrolling of glial fibrillary acidic protein, tumor necrosis factor-alpha, inwardly rectifying potassium channel (Kir 4.1) and aquaporin-4 in rat cerebral cortex |
| Authors: |
Dalia Mahmoud Abdelmonem Elsherbini, Fatma M. Ghoneim, Eman Mohammed El-Mancy, Hasnaa Ali Ebrahim, Mohamed El-Sherbiny, Mohamed El-Shafey, Rasha Hamed Al-Serwi, Nehal M. Elsherbiny |
| Source: |
Frontiers in Cellular Neuroscience, Vol 16 (2022) |
| Publisher Information: |
Frontiers Media S.A., 2022. |
| Publication Year: |
2022 |
| Collection: |
LCC:Neurosciences. Biological psychiatry. Neuropsychiatry |
| Subject Terms: |
GFAP, inwardly rectifying potassium channel, brain edema, aquaporin-4, TNFα, Neurosciences. Biological psychiatry. Neuropsychiatry, RC321-571 |
| More Details: |
Hepatic encephalopathy (HE) is a neurological disarray manifested as a sequel to chronic and acute liver failure (ALF). A potentially fatal consequence of ALF is brain edema with concomitant astrocyte enlargement. This study aims to outline the role of astrocytes in acute HE and shed light on the most critical mechanisms driving this role. Rats were allocated into two groups. Group 1, the control group, received the vehicle. Group 2, the TAA group, received TAA (300 mg/kg) for 3 days. Serum AST, ALT, and ammonia were determined. Liver and cerebral cortical sections were processed for hematoxylin and eosin staining. Additionally, mRNA expression and immunohistochemical staining of cortical GFAP, TNFα, Kir4.1, and AQP4 were performed. Cortical sections from the TAA group demonstrated neuropil vacuolation and astrocytes enlargement with focal gliosis. GFAP, TNFα, and AQP4 revealed increased mRNA expression, positive immunoreactivity, and a positive correlation to brain water content. In contrast, Kir 4.1 showed decreased mRNA expression and immunoreactivity and a negative correlation to brain water content. In conclusion, our findings revealed altered levels of TNFα, Kir 4.1, GFAP, and AQP4 in HE-associated brain edema. A more significant dysregulation of Kir 4.1 and TNFα was observed compared to AQP4 and GFAP. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1662-5102 |
| Relation: |
https://www.frontiersin.org/articles/10.3389/fncel.2022.896172/full; https://doaj.org/toc/1662-5102 |
| DOI: |
10.3389/fncel.2022.896172 |
| Access URL: |
https://doaj.org/article/4c35236ba0984f5f99eefa462b411273 |
| Accession Number: |
edsdoj.4c35236ba0984f5f99eefa462b411273 |
| Database: |
Directory of Open Access Journals |
