Bibliographic Details
| Title: |
The SmpB C-terminal tail helps tmRNA to recognize and enter stalled ribosomes |
| Authors: |
Mickey R. Miller, Allen R. Buskirk |
| Source: |
Frontiers in Microbiology, Vol 5 (2014) |
| Publisher Information: |
Frontiers Media S.A., 2014. |
| Publication Year: |
2014 |
| Collection: |
LCC:Microbiology |
| Subject Terms: |
SmpB, tmRNA, decoding, ribosome stalling, EFTu, Microbiology, QR1-502 |
| More Details: |
In bacteria, transfer-messenger RNA (tmRNA) and SmpB comprise the most common and effective system for rescuing stalled ribosomes. Ribosomes stall on mRNA transcripts lacking stop codons and are rescued as the defective mRNA is swapped for the tmRNA template in a process known as trans-translation. The tmRNA–SmpB complex is recruited to the ribosome independent of a codon–anticodon interaction. Given that the ribosome uses robust discriminatory mechanisms to select against non-cognate tRNAs during canonical decoding, it has been hard to explain how this can happen. Recent structural and biochemical studies show that SmpB licenses tmRNA entry through its interactions with the decoding center and mRNA channel. In particular, the C-terminal tail of SmpB promotes both EFTu activation and accommodation of tmRNA, the former through interactions with 16S rRNA nucleotide G530 and the latter through interactions with the mRNA channel downstream of the A site. Here we present a detailed model of the earliest steps in trans-translation, and in light of these mechanistic considerations, revisit the question of how tmRNA preferentially reacts with stalled, non-translating ribosomes. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1664-302X |
| Relation: |
http://journal.frontiersin.org/article/10.3389/fmicb.2014.00462/full; https://doaj.org/toc/1664-302X |
| DOI: |
10.3389/fmicb.2014.00462 |
| Access URL: |
https://doaj.org/article/4be75364e5f846a88c4851ec49eb51dc |
| Accession Number: |
edsdoj.4be75364e5f846a88c4851ec49eb51dc |
| Database: |
Directory of Open Access Journals |
