Bibliographic Details
| Title: |
Inhibition of Proliferation of 4T1 Breast Cancer Cells by Cuscuta Alcohol Extract Based on mTOR/HIF-1α/VEGF Signaling Pathway |
| Authors: |
Hui SONG, Xiaoqiao HUANG, Xiangming SUN, Yang HU, Wencai SHAN, Jing TANG, Wenlan LI |
| Source: |
Shipin gongye ke-ji, Vol 46, Iss 7, Pp 355-364 (2025) |
| Publisher Information: |
The editorial department of Science and Technology of Food Industry, 2025. |
| Publication Year: |
2025 |
| Collection: |
LCC:Food processing and manufacture |
| Subject Terms: |
cuscuta, breast cancer, transcriptomics, mtor/hif-1α/vegf signaling pathway, Food processing and manufacture, TP368-456 |
| More Details: |
Objective: To examine the inhibitory effects and mechanisms of Cuscuta alcohol extract on the proliferation of 4T1 breast cancer cells, both in vitro and in vivo. Methods: MTT, scratch, transwell, and flow cytometric assays were used to examine the effects of Cuscuta alcohol extract on the proliferation, migration, invasion, and apoptosis of 4T1 breast cancer cells in vitro. Additionally, an in vivo 4T1 tumor-bearing mouse model was established. ELISA kits measured TNF-α, IL-2, and IL-10 levels in 4T1 tumor-bearing mice after 14 days of intragastric Cuscuta alcohol extract administration. Concurrently, tumor tissues from both the model mice and the high-dose Cuscuta alcohol extract group were analyzed using transcriptome sequencing to identify differentially expressed genes. GO and KEGG enrichment analyses were conducted, and the Western Blot assays were performed to verify the expression of the mTOR/HIF-1α/VEGF signaling pathway. Results: Cuscuta alcohol extract (400, 800 and 1200 μg/mL) significantly reduced the proliferation, migration, and invasion of 4T1 breast cancer cells (P |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
Chinese |
| ISSN: |
1002-0306 |
| Relation: |
https://doaj.org/toc/1002-0306 |
| DOI: |
10.13386/j.issn1002-0306.2024050320 |
| Access URL: |
https://doaj.org/article/ac4badc4dd7e4dff9a9089366537f660 |
| Accession Number: |
edsdoj.4badc4dd7e4dff9a9089366537f660 |
| Database: |
Directory of Open Access Journals |
