Bibliographic Details
| Title: |
A pore-forming protein-induced surface-enhanced Raman spectroscopic strategy for dynamic tracing of cell membrane repair |
| Authors: |
Yuanjiao Yang, Yunlong Chen, Jingxing Guo, Huipu Liu, Huangxian Ju |
| Source: |
iScience, Vol 24, Iss 9, Pp 102980- (2021) |
| Publisher Information: |
Elsevier, 2021. |
| Publication Year: |
2021 |
| Collection: |
LCC:Science |
| Subject Terms: |
Membranes, Cell biology, Biophysics, Bioengineering, Science |
| More Details: |
Summary: The plasma membrane repair holds significance for maintaining cell survival and homeostasis. To achieve the sensitive visualization of membrane repair process for revealing its mechanism, this work designs a perforation-induced surface-enhanced Raman spectroscopy (SERS) strategy by conjugating Raman reporter (4-mercaptobenzoic acid) loaded gold nanostars with pore-forming protein streptolysin O (SLO) to induce the SERS signal on living cells. The SERS signal obviously decreases with the initiation of membrane repair and the degradation of SLO pores due to the departure of gold-nanostar-conjugated SLO. Thus, the designed strategy can dynamically visualize the complete cell membrane repair and provide a sensitive method to demonstrate the SLO endocytosis- and exocytosis-mediated repairing mechanism. Using DOX-resistant MCF-7 cells as a model, a timely repair-blocking technology for promoting the highly efficient treatment of drug-resistant cancer cells is also proposed. This work opens an avenue for probing the plasma membrane repairing mechanisms and designing the precision therapeutic schedule. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2589-0042 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S2589004221009482; https://doaj.org/toc/2589-0042 |
| DOI: |
10.1016/j.isci.2021.102980 |
| Access URL: |
https://doaj.org/article/4af8f0fb6e634ada8a28a7e2aaa64a77 |
| Accession Number: |
edsdoj.4af8f0fb6e634ada8a28a7e2aaa64a77 |
| Database: |
Directory of Open Access Journals |
