Macranthoidin B Modulates Key Metabolic Pathways to Enhance ROS Generation and Induce Cytotoxicity and Apoptosis in Colorectal Cancer
| Title: | Macranthoidin B Modulates Key Metabolic Pathways to Enhance ROS Generation and Induce Cytotoxicity and Apoptosis in Colorectal Cancer |
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| Authors: | Xing Fan, Jun Rao, Ziwei Zhang, Dengfeng Li, Wenhao Cui, Jun Zhang, Hua Wang, Fangfang Tou, Zhi Zheng, Qiang Shen |
| Source: | Cellular Physiology and Biochemistry, Vol 46, Iss 4, Pp 1317-1330 (2018) |
| Publisher Information: | Cell Physiol Biochem Press GmbH & Co KG, 2018. |
| Publication Year: | 2018 |
| Collection: | LCC:Physiology LCC:Biochemistry |
| Subject Terms: | Colorectal cancer, Macranthoidin B, Natural product, Metabolomics, Cancer therapy, Physiology, QP1-981, Biochemistry, QD415-436 |
| More Details: | Background/Aims: Induction of oxidative stress and reactive oxygen species (ROS) mediated-apoptosis have been utilized as effective strategies in anticancer therapy. Macranthoidin B (MB) is a potent inducer of ROS-mediated apoptosis in cancer, but its mechanism of action is poorly understood. Method: Superoxide production with MB exposure in colorectal cancer (CRC) cells was measured using lucigenin chemiluminescence and real-time PCR. MB’s inhibitory effect on proliferation and viability of CRC cells was determined by proliferation assays. MB’s effect on apoptosis of CRC cells was determined by Western blotting and annexin V-FITC/PI staining. MB’s effect on the growth of CRC xenografts in mice was assessed. An established metabolomics profiling platform combining ultra-performance liquid chromatography-tandem mass spectrometry (LC-MS) with gas chromatography-mass spectrometry (GC-MS) was performed to determine MB’s effect on total metabolite variation in CRC cells. Results: We found that MB increases ROS generation via modulating key metabolic pathways. Using metabolomics profiling platform combining LC-MS with GC-MS, a total of 236 metabolites were identified in HCT-116 cells in which 31 metabolites were determined to be significantly regulated (p ≤ 0.05) after MB exposure. A number of key metabolites revealed by metabolomics analysis include glucose, fructose, citrate, arginine, phenylalanine, and S-adenosylhomocysteine (SAH), suggesting specific modulation of metabolism on carbohydrates, amino acids and peptides, lipids, nucleotide, cofactors and vitamins in HCT-116 CRC cells with MB treatment highly associated with apoptosis triggered by enhanced ROS and activated caspase-3. Conclusion: Our results demonstrate that MB represses CRC cell proliferation by inducing ROS-mediated apoptosis. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 1015-8987 1421-9778 |
| Relation: | https://www.karger.com/Article/FullText/489147; https://doaj.org/toc/1015-8987; https://doaj.org/toc/1421-9778 |
| DOI: | 10.1159/000489147 |
| Access URL: | https://doaj.org/article/4ac4a8271fe34fd281983e28a1cd098f |
| Accession Number: | edsdoj.4ac4a8271fe34fd281983e28a1cd098f |
| Database: | Directory of Open Access Journals |
| ISSN: | 10158987 14219778 |
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| DOI: | 10.1159/000489147 |
| Published in: | Cellular Physiology and Biochemistry |
| Language: | English |