Bibliographic Details
| Title: |
Complementary methods for SARS-CoV-2 diagnosis in times of material shortage |
| Authors: |
Thaisa Lucas Sandri, Juliana Inoue, Johanna Geiger, Johanna-Marie Griesbaum, Constanze Heinzel, Michael Burnet, Rolf Fendel, Peter G. Kremsner, Jana Held, Andrea Kreidenweiss |
| Source: |
Scientific Reports, Vol 11, Iss 1, Pp 1-8 (2021) |
| Publisher Information: |
Nature Portfolio, 2021. |
| Publication Year: |
2021 |
| Collection: |
LCC:Medicine
LCC:Science |
| Subject Terms: |
Medicine, Science |
| More Details: |
Abstract The pandemic caused by SARS-CoV-2 resulted in increasing demands for diagnostic tests, leading to a shortage of recommended testing materials and reagents. This study reports on the performance of self-sampled alternative swabbing material (ordinary Q-tips tested against flocked swab and rayon swab), of reagents for classical RNA extraction (phenol/guanidine-based protocol against a commercial kit), and of intercalating dye-based one-step quantitative reverse transcription real-time PCRs (RT-qPCR) compared against the gold standard hydrolysis probe-based assays for SARS-CoV-2 detection. The study found sampling with Q-tips, RNA extraction with classical protocol and intercalating dye-based RT-qPCR as a reliable and comparably sensitive strategy for detection of SARS-CoV-2—particularly valuable in the current period with a resurgent and dramatic increase in SARS-CoV-2 infections and growing shortage of diagnostic materials especially for regions limited in resources. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2045-2322 |
| Relation: |
https://doaj.org/toc/2045-2322 |
| DOI: |
10.1038/s41598-021-91457-z |
| Access URL: |
https://doaj.org/article/e4a03982c6a84819bad572abfc336e46 |
| Accession Number: |
edsdoj.4a03982c6a84819bad572abfc336e46 |
| Database: |
Directory of Open Access Journals |
