Bibliographic Details
| Title: |
Regulatory network in heat stress response in parasitoid wasp focusing on Xap5 heat stress regulator |
| Authors: |
Shijiao Xiong, Kaili Yu, Haiwei Lin, Xinhai Ye, Shan Xiao, Yi Yang, David W. Stanley, Qisheng Song, Qi Fang, Gongyin Ye |
| Source: |
iScience, Vol 27, Iss 1, Pp 108622- (2024) |
| Publisher Information: |
Elsevier, 2024. |
| Publication Year: |
2024 |
| Collection: |
LCC:Science |
| Subject Terms: |
Biological sciences, Entomology, Genetics, Molecular genetics, Science |
| More Details: |
Summary: Insects are susceptible to elevated temperatures, resulting in impaired fertility, and shortened lifespan. This study investigated the genetic mechanisms underlying heat stress effects. We conducted RNA sequencing on Pteromalus puparum exposed to 25°C and 35°C, revealing transcriptional signatures. Weighted Gene Co-expression Network Analysis uncovered heat stress-associated modules, forming a regulatory network of 113 genes. The network is naturally divided into two subgroups, one linked to acute heat stress, including heat shock proteins (HSPs), and the other to chronic heat stress, involving lipogenesis genes. We identified an Xap5 Heat Shock Regulator (XHSR) gene as a crucial network component, validated through RNA interference and quantitative PCR assays. XHSR knockdown reduced wasps’ lifespan while directly inducing HSPs and mediating lipogenesis gene induction. CRISPR/Cas9-mediated knockout of the Drosophila XHSR homolog reduced mutants’ survival, highlighting its conserved role. This research sheds light on thermal tolerance mechanisms, offering potential applications in pest control amid global warming. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2589-0042 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S2589004223026998; https://doaj.org/toc/2589-0042 |
| DOI: |
10.1016/j.isci.2023.108622 |
| Access URL: |
https://doaj.org/article/499b9638bf164ccb9e9cf0124fa03d01 |
| Accession Number: |
edsdoj.499b9638bf164ccb9e9cf0124fa03d01 |
| Database: |
Directory of Open Access Journals |
