Academic Journal
Isolation and partial characterization of lytic phage against Salmonella Abortusequi
| Title: | Isolation and partial characterization of lytic phage against Salmonella Abortusequi |
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| Authors: | Harshit Verma, Divya Pramod, Mohd. Abbas, Awadhesh Prajapati, Durairajan Ramchandra, Mayank Rawat |
| Source: | Veterinary World, Vol 6, Iss 2.000, Pp 72-75 (2013) |
| Publisher Information: | Veterinary World, 2013. |
| Publication Year: | 2013 |
| Collection: | LCC:Animal culture LCC:Veterinary medicine |
| Subject Terms: | bacteriophage, lytic phage, Salmonella Abortusequi, Animal culture, SF1-1100, Veterinary medicine, SF600-1100 |
| More Details: | Aim: To use partially characterized phages for generation of phage lysates having potential to be developed as vaccines against Salmonella Abortusequi. Material and Methods: Phage/s isolation from several sewage samples was attempted against Salmonella Abortusequi strains procured from the National Salmonella Reference Centre, IVRI using enrichment and agar overlay technique. A concentrated stock of the phage was prepared after its purification. The lytic range, morphology and DNA size were determined. Results: Two lytic phages could be isolated and purified. The phage with wider lytic range was found to possess a hexagonal head with a very short tail. The DNA size was found to be 21kb. Conclusion: The investigation reports first isolation of a lytic phage against Salmonella Abortusequi. The phage can be used in future for development of phage lysate based immune therapeutic agent against equine abortions. [Vet World 2013; 6(2.000): 72-75] |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 0972-8988 2231-0916 |
| Relation: | http://www.scopemed.org/fulltextpdf.php?mno=21954; https://doaj.org/toc/0972-8988; https://doaj.org/toc/2231-0916 |
| DOI: | 10.5455/vetworld.2013.72-75 |
| Access URL: | https://doaj.org/article/adedd4915fcc46199e46780db273f780 |
| Accession Number: | edsdoj.4915fcc46199e46780db273f780 |
| Database: | Directory of Open Access Journals |
| ISSN: | 09728988 22310916 |
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| DOI: | 10.5455/vetworld.2013.72-75 |
| Published in: | Veterinary World |
| Language: | English |