Lauric acid reduces apoptosis by inhibiting FOXO3a-signaling in deoxynivalenol-treated IPEC-J2 cells
| Title: | Lauric acid reduces apoptosis by inhibiting FOXO3a-signaling in deoxynivalenol-treated IPEC-J2 cells |
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| Authors: | Na Yeon Kim, Sang In Lee |
| Source: | Journal of Animal Science and Technology, Vol 66, Iss 5, Pp 1010-1020 (2024) |
| Publisher Information: | Korean Society of Animal Sciences and Technology, 2024. |
| Publication Year: | 2024 |
| Collection: | LCC:Animal culture |
| Subject Terms: | Intestinal porcine epithelial cell line (IPEC-J2 cells), Deoxynivalenol, Lauric acid, Apoptosis, Foxo3a, Animal culture, SF1-1100 |
| More Details: | Deoxynivalenol (DON) is the most common mycotoxin contaminant of food or feed worldwide and causes disease in animals. Lauric acid (LA) is a medium-chain fatty acid useful for barrier functions such as antimicrobial activity in the intestine of monogastric animals. However, the molecular mechanisms by which lauric acid exerts its effects on the deoxynivalenol-exposed small intestine have not been studied. We used an intestinal porcine epithelial cell line (IPEC-J2) as an in vitro model to explore the molecular mechanism of lauric acid in alleviating deoxynivalenol-induced damage. We found that lauric acid reversed deoxynivalenol-induced reduction in cell viability. Our quantitative real-time polymerase chain reaction results indicated that lauric acid alleviated deoxynivalenol-induced apoptosis through Annexin-V. Additionally, immunofluorescence and Western blotting showed that lauric acid attenuated deoxynivalenol-induced forkhead box O3 (FOXO3a) translocation into the nucleus. These results suggest that lauric acid attenuates forkhead box O3 translocation in the small intestine damaged by deoxynivalenol, thereby reducing apoptosis. In conclusion, this study found that lauric acid alleviates deoxynivalenol-induced damage in intestinal porcine epithelial cell line through various molecular mechanisms. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 2672-0191 2055-0391 |
| Relation: | http://www.ejast.org/archive/view_article?doi=10.5187/jast.2023.e92; https://doaj.org/toc/2672-0191; https://doaj.org/toc/2055-0391 |
| DOI: | 10.5187/jast.2023.e92 |
| Access URL: | https://doaj.org/article/48eff5350082472d8e4cfd7dc2bded17 |
| Accession Number: | edsdoj.48eff5350082472d8e4cfd7dc2bded17 |
| Database: | Directory of Open Access Journals |
| ISSN: | 26720191 20550391 |
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| DOI: | 10.5187/jast.2023.e92 |
| Published in: | Journal of Animal Science and Technology |
| Language: | English |