Effect of the TGF-β/BMP Signaling Pathway on the Proliferation of Yak Pulmonary Artery Smooth Muscle Cells under Hypoxic Conditions

Bibliographic Details
Title: Effect of the TGF-β/BMP Signaling Pathway on the Proliferation of Yak Pulmonary Artery Smooth Muscle Cells under Hypoxic Conditions
Authors: Junfeng He, Kejin Wang, Biao Wang, Yan Cui, Qian Zhang
Source: Animals, Vol 14, Iss 14, p 2072 (2024)
Publisher Information: MDPI AG, 2024.
Publication Year: 2024
Collection: LCC:Veterinary medicine
LCC:Zoology
Subject Terms: yak, hypoxia, pulmonary artery smooth muscle cells, proliferation, Veterinary medicine, SF600-1100, Zoology, QL1-991
More Details: To survive in low-oxygen environments, yaks effectively avoid hypoxia-induced pulmonary arterial hypertension through vascular remodeling. The TGF-β/BMP signaling pathway plays a key role in maintaining the homeostasis of pulmonary artery smooth muscle cells (PASMCs). However, little is known about the molecular regulatory mechanisms by which the TGF-β/BMP signaling pathway contributes to the proliferation of yak PASMCs. In this study, yak PASMCs were cultured in vitro, and a hypoxia model was constructed to investigate the effect of TGFβ/BMP signaling on yak PASMC proliferation. Hypoxia treatment increased the proliferation of yak PASMCs significantly. As the duration of hypoxia increased, the expression levels of TGF-β1 and the phosphorylation levels of Smad2/3 were upregulated significantly. The BMP signaling pathway was transiently activated by hypoxia, with increases in BMPR2 expression and Smad1/5 phosphorylation, and these changes were gradually reversed with prolonged hypoxia exposure. In addition, exogenous TGF-β1 activated the TGF-β signaling pathway, increased the phosphorylation levels of the downstream proteins Smad2 and Smad3, and increased the proliferation and migration rates of yak PASMCs significantly. Finally, treatment with noggin (an inhibitor of BMP signaling) significantly reduced BMPR2 protein expression levels and Smad1/5 phosphorylation levels and increased yak PASMC proliferation and migration rates. In summary, these results revealed that under hypoxic conditions, the dynamic regulation of the TGF-β/BMP signaling pathway promotes the proliferation of yak PASMCs.
Document Type: article
File Description: electronic resource
Language: English
ISSN: 2076-2615
Relation: https://www.mdpi.com/2076-2615/14/14/2072; https://doaj.org/toc/2076-2615
DOI: 10.3390/ani14142072
Access URL: https://doaj.org/article/48b7a05874a5429084655d8be4b37515
Accession Number: edsdoj.48b7a05874a5429084655d8be4b37515
Database: Directory of Open Access Journals
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More Details
ISSN:20762615
DOI:10.3390/ani14142072
Published in:Animals
Language:English