Bibliographic Details
| Title: |
Distribution of bone-marrow-derived endothelial and immune cells in a murine colitis-associated colorectal cancer model. |
| Authors: |
Chuan-Xing Xiao, Huan-Huan Wang, Ying Shi, Ping Li, Yun-Peng Liu, Jian-Lin Ren, Bayasi Guleng |
| Source: |
PLoS ONE, Vol 8, Iss 9, p e73666 (2013) |
| Publisher Information: |
Public Library of Science (PLoS), 2013. |
| Publication Year: |
2013 |
| Collection: |
LCC:Medicine
LCC:Science |
| Subject Terms: |
Medicine, Science |
| More Details: |
Inflammatory bowel disease (IBD) can lead to an increased risk of developing colorectal cancer (CRC). The aim of this study was to establish a model for combined bone marrow transplantation (BMT) and colitis-associated colorectal cancer (CAC) and to define the contribution of BM-derived cells during the inflammation associated with carcinogenesis. We established a model for BMT using green fluorescent protein (GFP) transgenic mice, followed by AOM/DSS-induced CAC, and performed confocal microscopy analysis on in vivo living tissue and frozen tumor sections. Our imaging analyses showed that GFP-positive cells extensively infiltrated the tumor stroma and that some WGA and GFP or CD31 and GFP double-positive cells were observed in the lining of tumor vessels. Flow cytometry analysis of the tumor-infiltrating cells showed that the GFP-positive CD11c+ DCs cells were one-third of the GFP+/CD11C- cells, and that half of these DCs (0.96% vs 1.02%) were GFP-positive BM-derived cells. The majority of CD4(+) T cells were GFP-negative (12.02% vs 1.9%), and we discovered a novel CD4(+) CD11c(+) DC subset (0.34% vs 1.64%). In conclusion, we defined the distribution of BM-derived endothelial cells, CD11c(+) DCs and CD4(+) T cells in tumors. This model might be useful for elucidating the diverse BM-derived cell types and functions during the progression of colitis-associated colorectal cancer. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1932-6203 |
| Relation: |
http://europepmc.org/articles/PMC3769344?pdf=render; https://doaj.org/toc/1932-6203 |
| DOI: |
10.1371/journal.pone.0073666 |
| Access URL: |
https://doaj.org/article/484e9ccca45042d68256e0759793a529 |
| Accession Number: |
edsdoj.484e9ccca45042d68256e0759793a529 |
| Database: |
Directory of Open Access Journals |
