Academic Journal
In Vivo Resistance to Ceftolozane/Tazobactam in Pseudomonas aeruginosa Arising by AmpC- and Non-AmpC-Mediated Pathways
| Title: | In Vivo Resistance to Ceftolozane/Tazobactam in Pseudomonas aeruginosa Arising by AmpC- and Non-AmpC-Mediated Pathways |
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| Authors: | Erik Skoglund, Henrietta Abodakpi, Rafael Rios, Lorena Diaz, Elsa De La Cadena, An Q. Dinh, Javier Ardila, William R. Miller, Jose M. Munita, Cesar A. Arias, Vincent H. Tam, Truc T. Tran |
| Source: | Case Reports in Infectious Diseases, Vol 2018 (2018) |
| Publisher Information: | Wiley, 2018. |
| Publication Year: | 2018 |
| Collection: | LCC:Infectious and parasitic diseases |
| Subject Terms: | Infectious and parasitic diseases, RC109-216 |
| More Details: | Two pairs of ceftolozane/tazobactam susceptible/resistant P. aeruginosa were isolated from 2 patients after exposure to β-lactams. The genetic basis of ceftolozane/tazobactam resistance was evaluated, and β-lactam-resistant mechanisms were assessed by phenotypic assays. Whole genome sequencing identified mutations in AmpC including the mutation (V213A) and a deletion of 7 amino acids (P210–G216) in the Ω-loop. Phenotypic assays showed that ceftolozane/tazobactam resistance in the strain with AmpCV213A variant was associated with increased β-lactamase hydrolysis activity. On the other hand, the deletion of 7 amino acids in the Ω-loop of AmpC did not display enhanced β-lactamase activity. Resistance to ceftolozane/tazobactam in P. aeruginosa is associated with changes in AmpC; however, the apparent loss of β-lactamase activity in AmpC∆7 suggests that non-AmpC mechanisms could play an important role in resistance to β-lactam/β-lactamase inhibitor combinations. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 2090-6625 2090-6633 |
| Relation: | https://doaj.org/toc/2090-6625; https://doaj.org/toc/2090-6633 |
| DOI: | 10.1155/2018/9095203 |
| Access URL: | https://doaj.org/article/47ed234790a04dd889c2fbfca540c2a7 |
| Accession Number: | edsdoj.47ed234790a04dd889c2fbfca540c2a7 |
| Database: | Directory of Open Access Journals |
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| ISSN: | 20906625 20906633 |
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| DOI: | 10.1155/2018/9095203 |
| Published in: | Case Reports in Infectious Diseases |
| Language: | English |