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Genetic mapping of AhVt1, a novel genetic locus that confers the variegated testa color in cultivated peanut (Arachis hypogaea L.) and its utilization for marker-assisted selection

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Title: Genetic mapping of AhVt1, a novel genetic locus that confers the variegated testa color in cultivated peanut (Arachis hypogaea L.) and its utilization for marker-assisted selection
Authors: Hao Chen, Xinlei Yang, Rirong Xu, Xiangyu Chen, Haifeng Zhong, Nian Liu, Li Huang, Huaiyong Luo, Dongxin Huai, Wenjing Liu, Yuhua Chen, Jianhong Chen, Huifang Jiang
Source: Frontiers in Plant Science, Vol 14 (2023)
Publisher Information: Frontiers Media S.A., 2023.
Publication Year: 2023
Collection: LCC:Plant culture
Subject Terms: variegated testa color, BSA-seq, peanut (Arachis hypogaea L), genetic mapping, marker- assisted selection, Plant culture, SB1-1110
More Details: IntroductionPeanut (Arachis hypogaea L.) is an important cash crop worldwide. Compared with the ordinary peanut with pure pink testa, peanut with variegated testa color has attractive appearance and a higher market value. In addition, the variegated testa represents a distinct regulation pattern of anthocyanin accumulation in integument cells.MethodsIn order to identify the genetic locus underlying variegated testa color in peanut, two populations were constructed from the crosses between Fuhua 8 (pure-pink testa) and Wucai (red on white variegated testa), Quanhonghua 1 (pure-red testa) and Wucai, respectively. Genetic analysis and bulked sergeant analysis sequencing were applied to detect and identify the genetic locus for variegated testa color. Marker-assisted selection was used to develop new variegated testa peanut lines.ResultsAs a result, all the seeds harvested from the F1 individuals of both populations showed the variegated testa type with white trace. Genetic analysis revealed that the pigmentation of colored region in red on white variegated testa was controlled by a previous reported gene AhRt1, while the formation of white region (un-pigmented region) in variegated testa was controlled by another single genetic locus. This locus, named as AhVt1 (Arachis hypogaea Variegated Testa 1), was preliminary mapped on chromosome 08 through bulked sergeant analysis sequencing. Using a secondary mapping population derived from the cross between Fuhua 8 and Wucai, AhVt1 was further mapped to a 1.89-Mb genomic interval by linkage analysis, and several potential genes associated with the uneven distribution of anthocyanin, such as MADS-box, MYB, and Chalcone synthase-like protein, were harbored in the region. Moreover, the molecular markers closely linked to the AhVt1 were developed, and the new variegated testa peanut lines were obtained with the help of marker-assisted selection.ConclusionOur findings will accelerate the breeding program for developing new peanut varieties with “colorful” testa colors and laid a foundation for map-based cloning of gene responsible for variegated testa.
Document Type: article
File Description: electronic resource
Language: English
ISSN: 1664-462X
Relation: https://www.frontiersin.org/articles/10.3389/fpls.2023.1145098/full; https://doaj.org/toc/1664-462X
DOI: 10.3389/fpls.2023.1145098
Access URL: https://doaj.org/article/e47ae74104834f44b86d9d5ecdf74560
Accession Number: edsdoj.47ae74104834f44b86d9d5ecdf74560
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  Data: Genetic mapping of AhVt1, a novel genetic locus that confers the variegated testa color in cultivated peanut (Arachis hypogaea L.) and its utilization for marker-assisted selection
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  Data: <searchLink fieldCode="AR" term="%22Hao+Chen%22">Hao Chen</searchLink><br /><searchLink fieldCode="AR" term="%22Xinlei+Yang%22">Xinlei Yang</searchLink><br /><searchLink fieldCode="AR" term="%22Rirong+Xu%22">Rirong Xu</searchLink><br /><searchLink fieldCode="AR" term="%22Xiangyu+Chen%22">Xiangyu Chen</searchLink><br /><searchLink fieldCode="AR" term="%22Haifeng+Zhong%22">Haifeng Zhong</searchLink><br /><searchLink fieldCode="AR" term="%22Nian+Liu%22">Nian Liu</searchLink><br /><searchLink fieldCode="AR" term="%22Li+Huang%22">Li Huang</searchLink><br /><searchLink fieldCode="AR" term="%22Huaiyong+Luo%22">Huaiyong Luo</searchLink><br /><searchLink fieldCode="AR" term="%22Dongxin+Huai%22">Dongxin Huai</searchLink><br /><searchLink fieldCode="AR" term="%22Wenjing+Liu%22">Wenjing Liu</searchLink><br /><searchLink fieldCode="AR" term="%22Yuhua+Chen%22">Yuhua Chen</searchLink><br /><searchLink fieldCode="AR" term="%22Jianhong+Chen%22">Jianhong Chen</searchLink><br /><searchLink fieldCode="AR" term="%22Huifang+Jiang%22">Huifang Jiang</searchLink>
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  Data: IntroductionPeanut (Arachis hypogaea L.) is an important cash crop worldwide. Compared with the ordinary peanut with pure pink testa, peanut with variegated testa color has attractive appearance and a higher market value. In addition, the variegated testa represents a distinct regulation pattern of anthocyanin accumulation in integument cells.MethodsIn order to identify the genetic locus underlying variegated testa color in peanut, two populations were constructed from the crosses between Fuhua 8 (pure-pink testa) and Wucai (red on white variegated testa), Quanhonghua 1 (pure-red testa) and Wucai, respectively. Genetic analysis and bulked sergeant analysis sequencing were applied to detect and identify the genetic locus for variegated testa color. Marker-assisted selection was used to develop new variegated testa peanut lines.ResultsAs a result, all the seeds harvested from the F1 individuals of both populations showed the variegated testa type with white trace. Genetic analysis revealed that the pigmentation of colored region in red on white variegated testa was controlled by a previous reported gene AhRt1, while the formation of white region (un-pigmented region) in variegated testa was controlled by another single genetic locus. This locus, named as AhVt1 (Arachis hypogaea Variegated Testa 1), was preliminary mapped on chromosome 08 through bulked sergeant analysis sequencing. Using a secondary mapping population derived from the cross between Fuhua 8 and Wucai, AhVt1 was further mapped to a 1.89-Mb genomic interval by linkage analysis, and several potential genes associated with the uneven distribution of anthocyanin, such as MADS-box, MYB, and Chalcone synthase-like protein, were harbored in the region. Moreover, the molecular markers closely linked to the AhVt1 were developed, and the new variegated testa peanut lines were obtained with the help of marker-assisted selection.ConclusionOur findings will accelerate the breeding program for developing new peanut varieties with “colorful” testa colors and laid a foundation for map-based cloning of gene responsible for variegated testa.
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