| Title: |
α-Mangostin-phytosomes as a plausible nano-vesicular approach for enhancing cytotoxic activity on SKOV-3 ovarian cancer cells |
| Authors: |
Abdulmohsin J. Alamoudi, Shaimaa M. Badr-Eldin, Osama A. A. Ahmed, Serag Eldin I. Elbehairi, Mohammad Y. Alfaifi, Hani Z. Asfour, Gamal A. Mohamed, Sabrin R. M. Ibrahim, Ashraf B. Abdel-Naim, Hossam M. Abdallah |
| Source: |
Future Journal of Pharmaceutical Sciences, Vol 10, Iss 1, Pp 1-19 (2024) |
| Publisher Information: |
SpringerOpen, 2024. |
| Publication Year: |
2024 |
| Collection: |
LCC:Therapeutics. Pharmacology
LCC:Pharmacy and materia medica |
| Subject Terms: |
α-Mangostin, Garcinia mangostana, Phytosome, SKOV-3, Apoptosis, Caspase, Therapeutics. Pharmacology, RM1-950, Pharmacy and materia medica, RS1-441 |
| More Details: |
Abstract Background α-Mangostin is a major xanthone in Garcinia mangostana L. (Clusiaceae) pericarps. It has promising anti-proliferative potential in different cancer cells; however, it has poor oral bioavailability. Phytosomes are used as a novel nano-based drug delivery system. The aim of this research was to enhance the anti-proliferative potency of α-mangostin by formulating it as α-mangostin-phytosome (α-M-PTMs) and assessing its impact on SKOV-3 ovarian cancer cells in comparison to pure α-mangostin. Results The size and entrapment efficiency of the proposed formulation were optimized using Box–Behnken statistics. The optimized formula was characterized using transmission electron microscope. The binding of α-mangostin to phospholipids was confirmed using Fourier-transform infrared (FTIR) spectroscopy. The optimized α-mangostin-phytosomes formula exhibited enhanced anti-proliferative activity with reference to raw α-mangostin. This was further substantiated by assessing the cell cycle phases that indicated an accumulation of SKOV-3 cells in the sub-G1 phase. Annexin-V staining revealed enhanced apoptotic activity in α-mangostin-phytosome-treated cells. This was associated with upregulation of CASP3 (Caspase-3), BAX (BCL2 Associated X, Apoptosis Regulator) and TP53 as well as down-regulation of BCL2 mRNA (B-Cell Leukemia/Lymphoma 2). Moreover, our data indicated enhanced ROS (Reactive oxygen species) production, cytochrome-C release, and disturbed MMP (mitochondrial membrane potential). Conclusion Encapsulation of α-mangostin in a phytosome nano-formula enhances its anti-proliferative effects in SKOV-3 cells via, at least in part, inducing mitochondrial apoptotic cell death. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2314-7253 |
| Relation: |
https://doaj.org/toc/2314-7253 |
| DOI: |
10.1186/s43094-024-00718-x |
| Access URL: |
https://doaj.org/article/d474cdfb83cc4aa98c0baf6bc82d1a56 |
| Accession Number: |
edsdoj.474cdfb83cc4aa98c0baf6bc82d1a56 |
| Database: |
Directory of Open Access Journals |
