| Title: |
Activated gut-homing CD8+ T cells for coeliac disease diagnosis on a gluten-free diet |
| Authors: |
Fernando Fernández-Bañares, Natalia López-Palacios, María Corzo, Beatriz Arau, Mercedes Rubio, Marta Fernández-Prieto, Eva Tristán, Mar Pujals, Sergio Farrais, Saúl Horta, Juana María Hernández, Marta Gomez-Perosanz, Pedro A. Reche, María Esteve, Concepción Núñez |
| Source: |
BMC Medicine, Vol 19, Iss 1, Pp 1-10 (2021) |
| Publisher Information: |
BMC, 2021. |
| Publication Year: |
2021 |
| Collection: |
LCC:Medicine |
| Subject Terms: |
CD8 T cells, Celiac disease, Gluten challenge, Gluten-free diet, IFN-γ ELISPOT, Flow cytometry, Medicine |
| More Details: |
Abstract Background The diagnosis of coeliac disease (CD) in individuals that have started a gluten-free diet (GFD) without an adequate previous diagnostic work-out is a challenge. Several immunological assays such as IFN-γ ELISPOT have been developed to avoid the need of prolonged gluten challenge to induce the intestinal damage. We aimed to evaluate the diagnostic accuracy of activated gut-homing CD8+ and TCRγδ+ T cells in blood after a 3-day gluten challenge and to compare it with the performance of IFN-γ ELISPOT in a HLA-DQ2.5 subsample. Methods A total of 22 CD patients and 48 non-CD subjects, all of them following a GFD, underwent a 3-day 10-g gluten challenge. The percentage of two T cell subsets (CD8+ CD103+ β7hi CD38+/total CD8+ and TCRγδ+ CD103+ β7hi CD38+/total TCRγδ+) in fresh peripheral blood drawn baseline and 6 days after the challenge was determined by flow cytometry. IFN-γ ELISPOT assays were also performed in HLA-DQ2.5 participants. ROC curve analysis was used to assess the diagnostic performance of the CD8+ T cell response and IFN-γ ELISPOT. Results Significant differences between the percentage of the two studied subsets of CD8+ and TCRγδ+ cells at days 0 and 6 were found only when considering CD patients (p < 10−3 vs. non-CD subjects). Measuring activated CD8+ T cells provided accurate CD diagnosis with 95% specificity and 97% sensitivity, offering similar results than IFN-γ ELISPOT. Conclusions The results provide a highly accurate blood test for CD diagnosis in patients on a GFD of easy implementation in daily clinical practice. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1741-7015 |
| Relation: |
https://doaj.org/toc/1741-7015 |
| DOI: |
10.1186/s12916-021-02116-z |
| Access URL: |
https://doaj.org/article/45ad4a6b99cb4d7786e8ea95c49ba0e4 |
| Accession Number: |
edsdoj.45ad4a6b99cb4d7786e8ea95c49ba0e4 |
| Database: |
Directory of Open Access Journals |
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