Bibliographic Details
| Title: |
A novel mushroom (Auricularia polytricha) glycoprotein protects against lead-induced hepatoxicity, promotes lead adsorption, inhibits organ accumulation of lead, upregulates detoxifying proteins, and enhances immunoregulation in rats |
| Authors: |
Shuang Zhao, Yi Gao, Hexiang Wang, Yangyang Fan, Pan Wang, Wenting Zhao, Jack Ho Wong, Dan Wang, Xiaoyan Zhao, Tzi Bun Ng |
| Source: |
Frontiers in Nutrition, Vol 10 (2023) |
| Publisher Information: |
Frontiers Media S.A., 2023. |
| Publication Year: |
2023 |
| Collection: |
LCC:Nutrition. Foods and food supply |
| Subject Terms: |
Auricularia polytricha, detoxification, glycoprotein, lead elimination activity, proteomics, Nutrition. Foods and food supply, TX341-641 |
| More Details: |
IntroductionLead is a ubiquitous environmental and industrial pollutant. Its nonbiodegradable toxicity induces a plethora of human diseases. A novel bioactive glycoprotein containing 1.15% carbohydrate, with the ability of adsorbing lead and effecting detoxification, has been purified from Auricularia polytricha and designated as APL. Besides, its mechanisms related to regulation of hepatic metabolic derangements at the proteome level were analyzed in this study.MethodsChromatographic techniques were utilized to purify APL in the current study. For investigating the protective effects of APL, Sprague-Dawley rats were given daily intraperitoneal injections of lead acetate for establishment of an animal model, and different dosages of APL were gastrically irrigated for study of protection from lead detoxification. Liver samples were prepared for proteomic analyses to explore the detoxification mechanisms.Results and discussionThe detoxifying glycoprotein APL displayed unique molecular properties with molecular weight of 252-kDa, was isolated from fruiting bodies of the edible fungus A. polytricha. The serum concentrations of lead and the liver function biomarkers aspartate and alanine aminotransferases were significantly (p |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2296-861X |
| Relation: |
https://www.frontiersin.org/articles/10.3389/fnut.2023.1144346/full; https://doaj.org/toc/2296-861X |
| DOI: |
10.3389/fnut.2023.1144346 |
| Access URL: |
https://doaj.org/article/de45350ab7f548afbf2bc402fa903ace |
| Accession Number: |
edsdoj.45350ab7f548afbf2bc402fa903ace |
| Database: |
Directory of Open Access Journals |
