Optimization of the Preparation Process of Oyster Peptide by Enzymatic Hydrolysis and Its Effects on Testosterone Secretion and Oxidative Stress in Mouse Testicular Interstitial Cells
| Title: | Optimization of the Preparation Process of Oyster Peptide by Enzymatic Hydrolysis and Its Effects on Testosterone Secretion and Oxidative Stress in Mouse Testicular Interstitial Cells |
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| Authors: | Lu LIU, Jingfeng LI, Meng LAN, Dongbing LI, Kaiyue ZHANG, Yuelong WANG, Jiaming SHEN, Chunnan LI, Hui ZHANG, Jiaming SUN |
| Source: | Shipin gongye ke-ji, Vol 45, Iss 9, Pp 168-176 (2024) |
| Publisher Information: | The editorial department of Science and Technology of Food Industry, 2024. |
| Publication Year: | 2024 |
| Collection: | LCC:Food processing and manufacture |
| Subject Terms: | oyster protein enzymolysis peptides, response surface optimization, biomimetic enzymolysis, testosterone, oxidative stress, Food processing and manufacture, TP368-456 |
| More Details: | In order to explore the research and development of oyster in the field of medicine and food homology, response surface methodology was used to optimize the preparation process of oyster protease-depeptidase, and its effects on testosterone secretion and oxidative stress in mouse leydig cells were studied. Based on the investigation of hydrolysis degree as the evaluation index, a biomimetic enzymatic hydrolysis method was employed to optimize the preparation process of oyster protein enzymolysis peptides using response surface analysis, building upon the foundation of single-factor experiments. Simultaneously, a hydrogen peroxide (H2O2)-induced oxidative damage model was established using mouse testicular interstitial cells (TM3), and the effects of oyster protein enzymolysis peptides on testosterone (T) secretion and oxidative stress were investigated through assessments of cell viability, DAPI staining, testosterone secretion level, superoxide dismutase (SOD) activity, and malondialdehyde (MDA) content in TM3 cells. The results showed that the optimal enzymatic hydrolysis conditions for oyster protein enzymolysis peptides were as follows: Substrate-to-solvent ratio of 1:10 g/mL, gastric protease concentration of 1.1%, hydrolysis time of 1.0 h, pancreatic protease concentration of 2.1%, and hydrolysis time of 3.1 h. Under these conditions, the degree of hydrolysis was determined to be 39.43%±0.42%. Oyster protein enzymolysis peptides exhibited varying degrees of proliferative activity on H2O2-induced TM3 cells, significantly (P |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | Chinese |
| ISSN: | 1002-0306 35315512 |
| Relation: | https://doaj.org/toc/1002-0306 |
| DOI: | 10.13386/j.issn1002-0306.2023050205 |
| Access URL: | https://doaj.org/article/44da5e000ce54888b888639c35315512 |
| Accession Number: | edsdoj.44da5e000ce54888b888639c35315512 |
| Database: | Directory of Open Access Journals |
| ISSN: | 10020306 35315512 |
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| DOI: | 10.13386/j.issn1002-0306.2023050205 |
| Published in: | Shipin gongye ke-ji |
| Language: | Chinese |