Academic Journal
Erucin inhibits osteoclast formation via suppressing cell–cell fusion molecule DC-STAMP without influencing mineralization by osteoblasts
| Title: | Erucin inhibits osteoclast formation via suppressing cell–cell fusion molecule DC-STAMP without influencing mineralization by osteoblasts |
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| Authors: | Tomohiro Takagi, Hirofumi Inoue, Shungo Fujii, Nobuyuki Takahashi, Mariko Uehara |
| Source: | BMC Research Notes, Vol 15, Iss 1, Pp 1-6 (2022) |
| Publisher Information: | BMC, 2022. |
| Publication Year: | 2022 |
| Collection: | LCC:Medicine LCC:Biology (General) LCC:Science (General) |
| Subject Terms: | Erucin, Isothiocyanate, Osteoclast differentiation, Osteoclast cell fusion, DC-STAMP, Medicine, Biology (General), QH301-705.5, Science (General), Q1-390 |
| More Details: | Abstract Objective Erucin (ERN), an isothiocyanate, is derived from the vegetable arugula. Although ERN has antitumor and antioxidant activity, the effect of ERN on osteoclast and osteoblast differentiation is not well documented. In this study, we evaluated the effects of ERN on osteoclast and osteoblast differentiation in vitro. Results ERN significantly reduced the formation of 1α,25(OH)2D3-induced tartrate-resistant acid phosphatase (TRAP)-positive cells at non-cytotoxic concentrations. Furthermore, ERN downregulated the mRNA expression of osteoclast-associated genes, such as nuclear factor of activated T cells cytoplasmic-1, TRAP, and cathepsin K. In addition, ERN suppressed mRNA expression of dendritic cell specific transmembrane protein (DC-STAMP), which encodes cell–cell fusion. However, ERN did not affect mineralization by osteoblasts. Thus, our data suggest that ERN may attenuate osteoclastic bone resorption by inhibiting multinucleation of mononuclear pre-osteoclasts and by suppressing mRNA expression of DC-STAMP in bone marrow cells without influencing mineralization by osteoblasts. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 1756-0500 |
| Relation: | https://doaj.org/toc/1756-0500 |
| DOI: | 10.1186/s13104-022-05988-3 |
| Access URL: | https://doaj.org/article/e4487edba674456e89c6ef374b98e641 |
| Accession Number: | edsdoj.4487edba674456e89c6ef374b98e641 |
| Database: | Directory of Open Access Journals |
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| ISSN: | 17560500 |
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| DOI: | 10.1186/s13104-022-05988-3 |
| Published in: | BMC Research Notes |
| Language: | English |