Bibliographic Details
| Title: |
Effect of micro-CT acquisition parameters and individual analysis on the assessment of bone repair |
| Authors: |
Milena Suemi IRIE, Rubens SPIN-NETO, Lucas Henrique Souza TEIXEIRA, Gustavo Davi RABELO, Nayara Teixeira de Araújo REIS, Priscilla Barbosa Ferreira SOARES |
| Source: |
Brazilian Oral Research, Vol 37 (2023) |
| Publisher Information: |
Sociedade Brasileira de Pesquisa Odontológica, 2023. |
| Publication Year: |
2023 |
| Collection: |
LCC:Dentistry |
| Subject Terms: |
X-Ray Microtomography, Bone and Bones, Wound Healing, Models, Animal, Cancellous Bone, Dentistry, RK1-715 |
| More Details: |
Abstract This study aimed to investigate whether two acquisition parameters, voxel size and filter thickness, used in a micro-computed tomography (micro-CT) scan, together with the examiner’s experience, influence the outcome of bone repair analysis in an experimental model. Bone defects were created in rat tibiae and scanned using two voxel sizes of 6- or 12-µm and two aluminum filter thickness of 0.5- or 1-mm. Then, bone volume fraction (BV/TV) and trabecular thickness (Tb.Th) were analyzed twice by two groups of operators: experienced and inexperienced examiners. For BV/TV, no significant differences were found between scanning voxel sizes of 6 and 12 µm for the experienced examiners; however, for the inexperienced examiners, the analysis performed using a 12-µm voxel size resulted in higher BV/TV values (32.4 and 32.9) than those acquired using a 6-µm voxel size (25.4 and 24.8) (p < 0.05). For Tb.Th, no significant differences between the analyses performed by experienced and inexperienced groups were observed when using the 6-µm voxel size. However, inexperienced examiners’ analysis revealed higher Tb.Th values when using the 12-µm voxel size compared with 6 µm (0.05 vs. 0.03, p < 0.05). Filter thickness had no influence on the results of any group. In conclusion, voxel size and operator experience affected the measured Tb.Th and BV/TV of a region with new bone formation. Operator experience in micro-CT analysis is more critical for BV/TV than for Tb.Th, whereas voxel size significantly affects Tb.Th evaluation. Operators in the initial phases of research training should be calibrated for bone assessments. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1807-3107 |
| Relation: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242023000100282&tlng=en; https://doaj.org/toc/1807-3107 |
| DOI: |
10.1590/1807-3107bor-2023.vol37.0099 |
| Access URL: |
https://doaj.org/article/43e23945a8f14988bdf379962bd4b68b |
| Accession Number: |
edsdoj.43e23945a8f14988bdf379962bd4b68b |
| Database: |
Directory of Open Access Journals |
