Germ-free housing conditions do not affect aortic root and aortic arch lesion size of late atherosclerotic low-density lipoprotein receptor-deficient mice
| Title: | Germ-free housing conditions do not affect aortic root and aortic arch lesion size of late atherosclerotic low-density lipoprotein receptor-deficient mice |
|---|---|
| Authors: | Klytaimnistra Kiouptsi, Giulia Pontarollo, Hristo Todorov, Johannes Braun, Sven Jäckel, Thomas Koeck, Franziska Bayer, Cornelia Karwot, Angelica Karpi, Susanne Gerber, Yvonne Jansen, Philipp Wild, Wolfram Ruf, Andreas Daiber, Emiel Van Der Vorst, Christian Weber, Yvonne Döring, Christoph Reinhardt |
| Source: | Gut Microbes, Vol 11, Iss 6, Pp 1809-1823 (2020) |
| Publisher Information: | Taylor & Francis Group, 2020. |
| Publication Year: | 2020 |
| Collection: | LCC:Diseases of the digestive system. Gastroenterology |
| Subject Terms: | microbiota, germ-free, low-density lipoprotein receptor-deficient mouse, atherosclerosis, aortic root, aortic arch, macrophages, smooth muscle cells, inflammatory markers, age, lesion size, Diseases of the digestive system. Gastroenterology, RC799-869 |
| More Details: | The microbiota has been linked to the development of atherosclerosis, but the functional impact of these resident bacteria on the lesion size and cellular composition of atherosclerotic plaques in the aorta has never been experimentally addressed with the germ-free low-density lipoprotein receptor-deficient (Ldlr−/-) mouse atherosclerosis model. Here, we report that 16 weeks of high-fat diet (HFD) feeding of hypercholesterolemic Ldlr−/- mice at germ-free (GF) housing conditions did not impact relative aortic root plaque size, macrophage content, and necrotic core area. Likewise, we did not find changes in the relative aortic arch lesion size. However, late atherosclerotic GF Ldlr−/- mice had altered inflammatory plasma protein markers and reduced smooth muscle cell content in their atherosclerotic root plaques relative to CONV-R Ldlr−/- mice. Neither absolute nor relative aortic root or aortic arch plaque size correlated with age. Our analyses on GF Ldlr−/- mice did not reveal a significant contribution of the microbiota in late aortic atherosclerosis. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 1949-0976 1949-0984 19490976 |
| Relation: | https://doaj.org/toc/1949-0976; https://doaj.org/toc/1949-0984 |
| DOI: | 10.1080/19490976.2020.1767463 |
| Access URL: | https://doaj.org/article/43b76087b6c94eb3aa203a15779e95a7 |
| Accession Number: | edsdoj.43b76087b6c94eb3aa203a15779e95a7 |
| Database: | Directory of Open Access Journals |
| ISSN: | 19490976 19490984 |
|---|---|
| DOI: | 10.1080/19490976.2020.1767463 |
| Published in: | Gut Microbes |
| Language: | English |