Bibliographic Details
| Title: |
Human Infections with Borna Disease Virus 1 (BoDV-1) Primarily Lead to Severe Encephalitis: Further Evidence from the Seroepidemiological BoSOT Study in an Endemic Region in Southern Germany |
| Authors: |
Markus Bauswein, Lisa Eidenschink, Gertrud Knoll, Bernhard Neumann, Klemens Angstwurm, Saida Zoubaa, Markus J Riemenschneider, Benedikt M J Lampl, Matthias Pregler, Hans Helmut Niller, Jonathan Jantsch, André Gessner, Yvonne Eberhardt, Gunnar Huppertz, Torsten Schramm, Stefanie Kühn, Michael Koller, Thomas Drasch, Yvonne Ehrl, Bernhard Banas, Robert Offner, Barbara Schmidt, Miriam C. Banas |
| Source: |
Viruses, Vol 15, Iss 1, p 188 (2023) |
| Publisher Information: |
MDPI AG, 2023. |
| Publication Year: |
2023 |
| Collection: |
LCC:Microbiology |
| Subject Terms: |
Borna disease virus 1 (BoDV-1), epidemiology, encephalitis, diagnostics, ELISA, indirect immunofluorescence assay (iIFA), Microbiology, QR1-502 |
| More Details: |
More than 40 human cases of severe encephalitis caused by Borna disease virus 1 (BoDV-1) have been reported to German health authorities. In an endemic region in southern Germany, we conducted the seroepidemiological BoSOT study (“BoDV-1 after solid-organ transplantation”) to assess whether there are undetected oligo- or asymptomatic courses of infection. A total of 216 healthy blood donors and 280 outpatients after solid organ transplantation were screened by a recombinant BoDV-1 ELISA followed by an indirect immunofluorescence assay (iIFA) as confirmatory test. For comparison, 288 serum and 258 cerebrospinal fluid (CSF) samples with a request for tick-borne encephalitis (TBE) diagnostics were analyzed for BoDV-1 infections. ELISA screening reactivity rates ranged from 3.5% to 18.6% depending on the cohort and the used ELISA antigen, but only one sample of a patient from the cohort with requested TBE diagnostics was confirmed to be positive for anti-BoDV-1-IgG by iIFA. In addition, the corresponding CSF sample of this patient with a three-week history of severe neurological disease tested positive for BoDV-1 RNA. Due to the iIFA results, all other results were interpreted as false-reactive in the ELISA screening. By linear serological epitope mapping, cross-reactions with human and bacterial proteins were identified as possible underlying mechanism for the false-reactive ELISA screening results. In conclusion, no oligo- or asymptomatic infections were detected in the studied cohorts. Serological tests based on a single recombinant BoDV-1 antigen should be interpreted with caution, and an iIFA should always be performed in addition. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1999-4915 |
| Relation: |
https://www.mdpi.com/1999-4915/15/1/188; https://doaj.org/toc/1999-4915 |
| DOI: |
10.3390/v15010188 |
| Access URL: |
https://doaj.org/article/439e519543cc4e6abb19424416ae83ba |
| Accession Number: |
edsdoj.439e519543cc4e6abb19424416ae83ba |
| Database: |
Directory of Open Access Journals |
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