| Title: |
The Regulation of Bone Resorption in Tooth Formation and Eruption Processes in Mouse Alveolar Crest Devoid of Cathepsin K |
| Authors: |
Masayo Okaji, Hideaki Sakai, Eiko Sakai, Mitsue Shibata, Fumio Hashimoto, Yasuhiro Kobayashi, Noriaki Yoshida, Kuniaki Okamoto, Kenji Yamamoto, Yuzo Kato |
| Source: |
Journal of Pharmacological Sciences, Vol 91, Iss 4, Pp 285-294 (2003) |
| Publisher Information: |
Elsevier, 2003. |
| Publication Year: |
2003 |
| Collection: |
LCC:Therapeutics. Pharmacology |
| Subject Terms: |
Therapeutics. Pharmacology, RM1-950 |
| More Details: |
Osteoclastic bone resorption has recently been implicated in the tooth formation and eruption in alveolar bone. Cathepsin K (CK) is a cysteine proteinase expressed predominantly in osteoclasts and is believed to play a critical role in degradation of bone matrix proteins. Here we present evidence that the alveolar bone resorption is essential for the tooth formation and that eruption proceeds normally in CK-deficient (CK–/–) mice. Radiographic and histological analyses revealed that the alveolar bone from these animals had no significant abnormalities during the tooth development between 5 and 28 days after birth. The tooth crown was normally erupted through the alveolar bone layer at 28 days after birth. The number of tartrate-resistant acid phosphatase-positive multinuclear cells in the alveolar bone around the tooth germ was apparently increased in 5-day-old CK–/– mice compared with age-matched littermates. More important, however, the immunohistochemical localization of matrix metalloproteinase-9 (MMP-9) was clearly increased in the CK–/– osteoclasts. In contrast, no significant difference in the immunoreactivity for cathepsin D was observed between the CK–/– osteoclasts and the wild-type ones. These results indicate that CK–/– osteoclasts are fully differentiated and are capable of degrading the organic phase of alveolar bone during the tooth formation and eruption, which may result from the compensatory action by MMP-9 increasingly expressed in the osteoclasts. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1347-8613 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S1347861319327112; https://doaj.org/toc/1347-8613 |
| DOI: |
10.1254/S1347-8613(19)32711-2 |
| Access URL: |
https://doaj.org/article/433e69953e6749f58d975ba468a4cf05 |
| Accession Number: |
edsdoj.433e69953e6749f58d975ba468a4cf05 |
| Database: |
Directory of Open Access Journals |
