Effect of Basic Fibroblast Growth Factor on Expression of Let-7 MicroRNA in Proliferation of Human Dental Pulp Cells
| Title: | Effect of Basic Fibroblast Growth Factor on Expression of Let-7 MicroRNA in Proliferation of Human Dental Pulp Cells |
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| Authors: | Pimporn Jirawechwongsakul, Patrayu Taebunpakul, Prasit Pavasant |
| Source: | Journal of Health Science and Medical Research (JHSMR), Vol 40, Iss 6, Pp 647-656 (2022) |
| Publisher Information: | Prince of Songkla University, 2022. |
| Publication Year: | 2022 |
| Collection: | LCC:Medicine |
| Subject Terms: | basic fibroblast growth factor, cell cycle, cell proliferation, human dental pulp cells, let-7 microrna, Medicine |
| More Details: | Objective: Basic fibroblast growth factor (bFGF) plays a pivotal role in cell proliferation, differentiation and extracellular matrix turnover in various tissues. In human dental pulp cells (HDPCs), let-7 microRNA is involved in cell proliferation and differentiation. There is little information on the effect of bFGF-induced cell proliferation on let-7 microRNA in HDPCs. This study investigated the effect of bFGF on let-7g, let-7f, and let-7i microRNAs and some of the genes involved in cell proliferation including p53 and Ki67 in HDPCs. Material and Methods: HDPCs were cultured and treated with bFGF at 0, 1, and 5 ng/mL. Cell proliferation was examined using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay at 24 and 48 hours. Additionally, gene expressions of let-7g, let-7f, let-7i microRNAs, and p53 and Ki67 were examined by quantitative real-time polymerase chain reaction at 24 hours. All experiments were performed in triplicate. Results: The results showed that let-7g, let-7f, and let-7i microRNAs were expressed in HDPCs. MTT assays showed that bFGF induced greater cell proliferation than the controls at 24 and 48 hours (p-value0.050). Conclusion: Our preliminary study showed that exogenous bFGF could decrease let-7g microRNA expression suggesting that let-7g microRNA may be involved in bFGF-induced HDPCs proliferation. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 2586-9981 2630-0559 |
| Relation: | https://www.jhsmr.org/index.php/jhsmr/article/view/876; https://doaj.org/toc/2586-9981; https://doaj.org/toc/2630-0559 |
| DOI: | 10.31584/jhsmr.2022876 |
| Access URL: | https://doaj.org/article/d43144be768a47b7955e29c2788ce236 |
| Accession Number: | edsdoj.43144be768a47b7955e29c2788ce236 |
| Database: | Directory of Open Access Journals |
| ISSN: | 25869981 26300559 |
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| DOI: | 10.31584/jhsmr.2022876 |
| Published in: | Journal of Health Science and Medical Research (JHSMR) |
| Language: | English |