Bibliographic Details
| Title: |
Rapid and Reliable Detection of SARS-CoV-2 Using Direct RT-LAMP |
| Authors: |
Sherif A. El-Kafrawy, Mai M. El-Daly, Ahmed M. Hassan, Steve M. Harakeh, Thamir A. Alandijany, Esam I. Azhar |
| Source: |
Diagnostics, Vol 12, Iss 4, p 828 (2022) |
| Publisher Information: |
MDPI AG, 2022. |
| Publication Year: |
2022 |
| Collection: |
LCC:Medicine (General) |
| Subject Terms: |
RT-LAMP, direct assay, SARS-CoV-2, COVID-19, molecular assay, PCR, Medicine (General), R5-920 |
| More Details: |
Background: The global pandemic coronavirus SARS-CoV-2 has a healthcare, social and economic burden. To limit the spread of the virus, the World Health Organization (WHO) urgently called for extensive screening of suspected individuals; thus, a quick, simple, and sensitive diagnostic assay is always in need. Methods: We applied reverse transcription-loop-mediated isothermal amplification (RT-LAMP) for the detection of SARS-CoV-2. The RT-LAMP method was optimized by evaluating two fluorescence amplification mixes and several reaction times, and results were compared to the standard real-time RT-PCR (rtRT-PCR). The assay was validated using 200 nasopharyngeal swabs collected in viral transport media (62 positive for SARS-CoV-2, and 138 negative for SARS-CoV-2 detected by the rtRT-PCR method). The samples were diluted 1:4 in diethylpyrocarbonate (DEPC)-treated water, utilized for RT-LAMP using different singleplex and multiplex sets of LAMP primers (N gene, S gene, and orf1ab gene), and incubated at 65 °C using real-time PCR 7500. Results: Our direct detection with the RT-LAMP protocol showed 100% concordance (sensitivity and specificity) with the standard protocol used for the detection of SARS-CoV-2 nucleic acid. Conclusions: In this study, we set up a rapid, simple, and sensitive RT-LAMP assay for the detection of SARS-CoV-2 in clinical samples. The assay is suitable for point of care detection in public hospitals, medical centers in rural areas, and in transportation hubs. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2075-4418 |
| Relation: |
https://www.mdpi.com/2075-4418/12/4/828; https://doaj.org/toc/2075-4418 |
| DOI: |
10.3390/diagnostics12040828 |
| Access URL: |
https://doaj.org/article/42c2f0d1667e4f94a17a5dd945910c2f |
| Accession Number: |
edsdoj.42c2f0d1667e4f94a17a5dd945910c2f |
| Database: |
Directory of Open Access Journals |
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