Bibliographic Details
| Title: |
Aglycone sterics-selective enzymatic glycan remodeling |
| Authors: |
Anwen Mao, Yan Zhang, Guyu Wang, Tong Zhong, Xinyu Chen, Haiqi Wang, Ran Xie, Xiaojian Wang, Lin Ding, Huangxian Ju |
| Source: |
iScience, Vol 25, Iss 7, Pp 104578- (2022) |
| Publisher Information: |
Elsevier, 2022. |
| Publication Year: |
2022 |
| Collection: |
LCC:Science |
| Subject Terms: |
Biological sciences, Biochemistry, Glycobiology, Enzyme engineering, Science |
| More Details: |
Summary: Precision remodeling of glycans in their native environments is pivotal for understanding glycan-mediated biological events and has important biotechnological implications in fields of clinical diagnosis, glyco-immune checkpoint therapy, and so forth. However, the influence of aglycone-steric diversity on the selectivity of glycan remodeling has been largely overlooked, limiting the application in complex biological scenarios. Here, we report the achievement of aglycone sterics-selective enzymatic glycan remodeling by controlled grafting of functional polymers from glycoenzyme. Through tuning polymer length, a series of enzyme-polymer composites with varying substrate permeability are prepared, which afford an activity pattern-based differentiation strategy for aglycone sterics. This leads to the implementation of glycolipid’s partner screening, and aglycone sterics-selective glycan remodeling in a complex biological environment. We further orchestrate the polymer length adjustment with external cues to regulate aglycone-steric selectivity in a multi-faceted fashion, resulting in an unexpected enhancement of glycolipid remodeling, and temporal control of glycan remodeling on live cells. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2589-0042 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S2589004222008501; https://doaj.org/toc/2589-0042 |
| DOI: |
10.1016/j.isci.2022.104578 |
| Access URL: |
https://doaj.org/article/ce4297db10fd413bafb1599d70fd3100 |
| Accession Number: |
edsdoj.4297db10fd413bafb1599d70fd3100 |
| Database: |
Directory of Open Access Journals |
