Guidelines for optimized gene knockout using CRISPR/Cas9

Bibliographic Details
Title:	Guidelines for optimized gene knockout using CRISPR/Cas9
Authors:	Claude Van Campenhout, Pauline Cabochette, Anne-Clémence Veillard, Miklos Laczik, Agnieszka Zelisko-Schmidt, Céline Sabatel, Maxime Dhainaut, Benoit Vanhollebeke, Cyril Gueydan, Véronique Kruys
Source:	BioTechniques, Vol 66, Iss 6, Pp 295-302 (2019)
Publisher Information:	Taylor & Francis Group, 2019.
Publication Year:	2019
Collection:	LCC:Biology (General)
Subject Terms:	gene targeting, methodology, quality control, Biology (General), QH301-705.5
More Details:	CRISPR/Cas9 technology has evolved as the most powerful approach to generate genetic models both for fundamental and preclinical research. Despite its apparent simplicity, the outcome of a genome-editing experiment can be substantially impacted by technical parameters and biological considerations. Here, we present guidelines and tools to optimize CRISPR/Cas9 genome-targeting efficiency and specificity. The nature of the target locus, the design of the single guide RNA and the choice of the delivery method should all be carefully considered prior to a genome-editing experiment. Different methods can also be used to detect off-target cleavages and decrease the risk of unwanted mutations. Together, these optimized tools and proper controls are essential to the assessment of CRISPR/Cas9 genome-editing experiments.
Document Type:	article
File Description:	electronic resource
Language:	English
ISSN:	2018-0187 1940-9818 0736-6205
Relation:	https://doaj.org/toc/0736-6205; https://doaj.org/toc/1940-9818
DOI:	10.2144/btn-2018-0187
Access URL:	https://doaj.org/article/a41ed023dfcf428ab64f10abd51ac30f
Accession Number:	edsdoj.41ed023dfcf428ab64f10abd51ac30f
Database:	Directory of Open Access Journals

More Details
ISSN:	20180187 19409818 07366205
DOI:	10.2144/btn-2018-0187
Published in:	BioTechniques
Language:	English