Bibliographic Details
| Title: |
Rapid and visual detection of novel astroviruses causing fatal gout in goslings using one-step reverse transcription loop-mediated isothermal amplification |
| Authors: |
Jun Ji, Qinxi Chen, Chaoge Sui, Wen Hu, Zhengli Yu, Zhibin Zhang, Xinhao Mu, Xin Xu, Lunguang Yao, Yunchao Kan, Qingmei Xie |
| Source: |
Poultry Science, Vol 99, Iss 9, Pp 4259-4264 (2020) |
| Publisher Information: |
Elsevier, 2020. |
| Publication Year: |
2020 |
| Collection: |
LCC:Animal culture |
| Subject Terms: |
N-GoAstV, rapidity, visible detection, one-step RT-LAMP, Animal culture, SF1-1100 |
| More Details: |
To visually and rapidly detect a novel goose astrovirus (N-GoAstV) causing fatal gout in goslings, an isothermal detection method based on one-step reverse transcription loop-mediated isothermal amplification (one-step RT-LAMP) was established. The one-step RT-LAMP assay for N-GoAstV detection, using Bst 3.0 DNA polymerase with strong reverse transcription activity and primer sets targeting the opening reading frame 1b (ORF1b) of N-GoAstV, could be completed in 30 min using a water bath at 61°C; the detection results could be visually observed by adding a pH-sensitive dye containing phenol red and cresol red. The detection limit of the one-step RT-LAMP assay was 57.8 copies, which was similar to that of reverse transcription–quantitative polymerase chain reaction. The assay specifically detected N-GoAstV without any cross-reaction with other reference viruses, and this was further confirmed using enzyme digestion. These results indicated that the newly established RT-LAMP assay could accomplish reverse transcription, amplification, and visual result determination in one step, and the results obtained via this rapid and cost-effective method could be used to support disease control on farms in terms of N-GoAstV infection. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
0032-5791 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S003257912030328X; https://doaj.org/toc/0032-5791 |
| DOI: |
10.1016/j.psj.2020.05.024 |
| Access URL: |
https://doaj.org/article/4158f644657c4556b1cc0e969d5bc513 |
| Accession Number: |
edsdoj.4158f644657c4556b1cc0e969d5bc513 |
| Database: |
Directory of Open Access Journals |
