Academic Journal
Activin/Nodal Signaling Supports Retinal Progenitor Specification in a Narrow Time Window during Pluripotent Stem Cell Neuralization
| Title: | Activin/Nodal Signaling Supports Retinal Progenitor Specification in a Narrow Time Window during Pluripotent Stem Cell Neuralization |
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| Authors: | Michele Bertacchi, Giuseppe Lupo, Luca Pandolfini, Simona Casarosa, Mara D’Onofrio, Roger A. Pedersen, William A. Harris, Federico Cremisi |
| Source: | Stem Cell Reports, Vol 5, Iss 4, Pp 532-545 (2015) |
| Publisher Information: | Elsevier, 2015. |
| Publication Year: | 2015 |
| Collection: | LCC:Medicine (General) LCC:Biology (General) |
| Subject Terms: | Medicine (General), R5-920, Biology (General), QH301-705.5 |
| More Details: | Retinal progenitors are initially found in the anterior neural plate region known as the eye field, whereas neighboring areas undertake telencephalic or hypothalamic development. Eye field cells become specified by switching on a network of eye field transcription factors, but the extracellular cues activating this network remain unclear. In this study, we used chemically defined media to induce in vitro differentiation of mouse embryonic stem cells (ESCs) toward eye field fates. Inhibition of Wnt/β-catenin signaling was sufficient to drive ESCs to telencephalic, but not retinal, fates. Instead, retinal progenitors could be generated from competent differentiating mouse ESCs by activation of Activin/Nodal signaling within a narrow temporal window corresponding to the emergence of primitive anterior neural progenitors. Activin also promoted eye field gene expression in differentiating human ESCs. Our results reveal insights into the mechanisms of eye field specification and open new avenues toward the generation of retinal progenitors for translational medicine. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 2213-6711 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2213671115002453; https://doaj.org/toc/2213-6711 |
| DOI: | 10.1016/j.stemcr.2015.08.011 |
| Access URL: | https://doaj.org/article/3e650a27161a4010b717039edbea4010 |
| Accession Number: | edsdoj.3e650a27161a4010b717039edbea4010 |
| Database: | Directory of Open Access Journals |
| ISSN: | 22136711 |
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| DOI: | 10.1016/j.stemcr.2015.08.011 |
| Published in: | Stem Cell Reports |
| Language: | English |