Bibliographic Details
| Title: |
Swift and portable detection of soybean mosaic virus SC7 through RNA extraction and loop-mediated isothermal amplification using lateral flow device |
| Authors: |
Shui-Xian Guo, Qing Zhang, Nan-Nan Bai, Pei-Yao Yue, Jing-Ping Niu, Cong-Cong Yin, Ai-Qin Yue, Wei-Jun Du, Jin-Zhong Zhao |
| Source: |
Frontiers in Microbiology, Vol 15 (2025) |
| Publisher Information: |
Frontiers Media S.A., 2025. |
| Publication Year: |
2025 |
| Collection: |
LCC:Microbiology |
| Subject Terms: |
soybean, soybean mosaic virus, virulent strain SC7, magnetic beads-based RNA extraction method, LAMP, lateral flow device, Microbiology, QR1-502 |
| More Details: |
The soybean mosaic disease—caused by the soybean mosaic virus (SMV)—significantly impacts soybean quality and yield. Among its various strains, SMV-SC7 is prevalent in China. Therefore, rapid and accurate diagnosis is deemed critical to mitigate the spread of SMV-SC7. In this study, a simple and rapid magnetic bead-based RNA extraction method was optimized. Furthermore, a reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay that requires no specialized equipment such as PCR Amplifier was proposed, employing a lateral flow device (LFD) for visual interpretation of SMV-SC7. The RT-LAMP-LFD approach facilitated specificity testing of SMV-SC7. Moreover, the limit of detection (LOD) of this method was as low as 10−5 ng (2.4 copies). The sensitivity of RT-LAMP-LFD was 10-fold higher than that of the colorimetric RT-LAMP method. In 194 field samples tested, the RT-LAMP-LFD detection of the SMV-SC7 had accuracy of 98.45% in comparison to RT-qPCR. In conclusion, the assay exhibited high specificity, sensitivity, and rapidity, enabling economical and portable detection of SMV-SC7 and providing technical support to identify SMV-SC7-infected soybeans. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1664-302X |
| Relation: |
https://www.frontiersin.org/articles/10.3389/fmicb.2024.1478218/full; https://doaj.org/toc/1664-302X |
| DOI: |
10.3389/fmicb.2024.1478218 |
| Access URL: |
https://doaj.org/article/c3d97867fa5445d4b9227a880242a409 |
| Accession Number: |
edsdoj.3d97867fa5445d4b9227a880242a409 |
| Database: |
Directory of Open Access Journals |
