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Spirobenzofuran Mitigates Ochratoxin A-Mediated Intestinal Adverse Effects in Pigs through Regulation of Beta Defensin 1

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Title: Spirobenzofuran Mitigates Ochratoxin A-Mediated Intestinal Adverse Effects in Pigs through Regulation of Beta Defensin 1
Authors: Jung Woong Yoon, Myoung Ok Kim, Sangsu Shin, Woo-Sung Kwon, Soo Hyun Kim, Yun-Ju Kwon, Sang In Lee
Source: Toxics, Vol 12, Iss 7, p 487 (2024)
Publisher Information: MDPI AG, 2024.
Publication Year: 2024
Collection: LCC:Chemical technology
Subject Terms: Ochratoxin A, IPEC-J2 cells, DEFB1 overexpression cell line, natural products, high-throughput screening, Spirobenzofuran, Chemical technology, TP1-1185
More Details: Antimicrobial peptides (AMPs) function to extensively suppress various problematic factors and are considered a new alternative for improving livestock health and enhancing immunomodulation. In this study, we explored whether AMP regulation has positive influences on Ochratoxin A (OTA) exposure using a porcine intestinal epithelial cell line (IPEC-J2 cells). We constructed a beta-defensin 1 (DEFB1) expression vector and used it to transfection IPEC-J2 cells to construct AMP overexpression cell lines. The results showed that OTA induced cytotoxicity, decreased cell migration, and increased inflammatory markers mRNA in IPEC-J2 cells. In DEFB1 overexpressing cell lines, OTA-induced reduced cell migration and increased inflammatory markers mRNA were alleviated. Additionally, a natural product capable of inducing DEFB1 expression, which was selected through high-throughput screening, showed significant alleviation of cytotoxicity, cell migration, and inflammatory markers compared to OTA-treated IPEC-J2 cells. Our finding provides novel insights and clues for the porcine industry, which is affected by OTA exposure.
Document Type: article
File Description: electronic resource
Language: English
ISSN: 2305-6304
Relation: https://www.mdpi.com/2305-6304/12/7/487; https://doaj.org/toc/2305-6304
DOI: 10.3390/toxics12070487
Access URL: https://doaj.org/article/3d25c2a5494d475ea92e70c8b2287ff4
Accession Number: edsdoj.3d25c2a5494d475ea92e70c8b2287ff4
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  Data: Spirobenzofuran Mitigates Ochratoxin A-Mediated Intestinal Adverse Effects in Pigs through Regulation of Beta Defensin 1
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  Data: <searchLink fieldCode="AR" term="%22Jung+Woong+Yoon%22">Jung Woong Yoon</searchLink><br /><searchLink fieldCode="AR" term="%22Myoung+Ok+Kim%22">Myoung Ok Kim</searchLink><br /><searchLink fieldCode="AR" term="%22Sangsu+Shin%22">Sangsu Shin</searchLink><br /><searchLink fieldCode="AR" term="%22Woo-Sung+Kwon%22">Woo-Sung Kwon</searchLink><br /><searchLink fieldCode="AR" term="%22Soo+Hyun+Kim%22">Soo Hyun Kim</searchLink><br /><searchLink fieldCode="AR" term="%22Yun-Ju+Kwon%22">Yun-Ju Kwon</searchLink><br /><searchLink fieldCode="AR" term="%22Sang+In+Lee%22">Sang In Lee</searchLink>
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  Data: Toxics, Vol 12, Iss 7, p 487 (2024)
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  Data: MDPI AG, 2024.
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  Data: <searchLink fieldCode="DE" term="%22Ochratoxin+A%22">Ochratoxin A</searchLink><br /><searchLink fieldCode="DE" term="%22IPEC-J2+cells%22">IPEC-J2 cells</searchLink><br /><searchLink fieldCode="DE" term="%22DEFB1+overexpression+cell+line%22">DEFB1 overexpression cell line</searchLink><br /><searchLink fieldCode="DE" term="%22natural+products%22">natural products</searchLink><br /><searchLink fieldCode="DE" term="%22high-throughput+screening%22">high-throughput screening</searchLink><br /><searchLink fieldCode="DE" term="%22Spirobenzofuran%22">Spirobenzofuran</searchLink><br /><searchLink fieldCode="DE" term="%22Chemical+technology%22">Chemical technology</searchLink><br /><searchLink fieldCode="DE" term="%22TP1-1185%22">TP1-1185</searchLink>
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  Data: Antimicrobial peptides (AMPs) function to extensively suppress various problematic factors and are considered a new alternative for improving livestock health and enhancing immunomodulation. In this study, we explored whether AMP regulation has positive influences on Ochratoxin A (OTA) exposure using a porcine intestinal epithelial cell line (IPEC-J2 cells). We constructed a beta-defensin 1 (DEFB1) expression vector and used it to transfection IPEC-J2 cells to construct AMP overexpression cell lines. The results showed that OTA induced cytotoxicity, decreased cell migration, and increased inflammatory markers mRNA in IPEC-J2 cells. In DEFB1 overexpressing cell lines, OTA-induced reduced cell migration and increased inflammatory markers mRNA were alleviated. Additionally, a natural product capable of inducing DEFB1 expression, which was selected through high-throughput screening, showed significant alleviation of cytotoxicity, cell migration, and inflammatory markers compared to OTA-treated IPEC-J2 cells. Our finding provides novel insights and clues for the porcine industry, which is affected by OTA exposure.
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        Value: 10.3390/toxics12070487
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        StartPage: 487
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      – SubjectFull: Ochratoxin A
        Type: general
      – SubjectFull: IPEC-J2 cells
        Type: general
      – SubjectFull: DEFB1 overexpression cell line
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      – TitleFull: Spirobenzofuran Mitigates Ochratoxin A-Mediated Intestinal Adverse Effects in Pigs through Regulation of Beta Defensin 1
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