Bibliographic Details
| Title: |
Spirobenzofuran Mitigates Ochratoxin A-Mediated Intestinal Adverse Effects in Pigs through Regulation of Beta Defensin 1 |
| Authors: |
Jung Woong Yoon, Myoung Ok Kim, Sangsu Shin, Woo-Sung Kwon, Soo Hyun Kim, Yun-Ju Kwon, Sang In Lee |
| Source: |
Toxics, Vol 12, Iss 7, p 487 (2024) |
| Publisher Information: |
MDPI AG, 2024. |
| Publication Year: |
2024 |
| Collection: |
LCC:Chemical technology |
| Subject Terms: |
Ochratoxin A, IPEC-J2 cells, DEFB1 overexpression cell line, natural products, high-throughput screening, Spirobenzofuran, Chemical technology, TP1-1185 |
| More Details: |
Antimicrobial peptides (AMPs) function to extensively suppress various problematic factors and are considered a new alternative for improving livestock health and enhancing immunomodulation. In this study, we explored whether AMP regulation has positive influences on Ochratoxin A (OTA) exposure using a porcine intestinal epithelial cell line (IPEC-J2 cells). We constructed a beta-defensin 1 (DEFB1) expression vector and used it to transfection IPEC-J2 cells to construct AMP overexpression cell lines. The results showed that OTA induced cytotoxicity, decreased cell migration, and increased inflammatory markers mRNA in IPEC-J2 cells. In DEFB1 overexpressing cell lines, OTA-induced reduced cell migration and increased inflammatory markers mRNA were alleviated. Additionally, a natural product capable of inducing DEFB1 expression, which was selected through high-throughput screening, showed significant alleviation of cytotoxicity, cell migration, and inflammatory markers compared to OTA-treated IPEC-J2 cells. Our finding provides novel insights and clues for the porcine industry, which is affected by OTA exposure. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2305-6304 |
| Relation: |
https://www.mdpi.com/2305-6304/12/7/487; https://doaj.org/toc/2305-6304 |
| DOI: |
10.3390/toxics12070487 |
| Access URL: |
https://doaj.org/article/3d25c2a5494d475ea92e70c8b2287ff4 |
| Accession Number: |
edsdoj.3d25c2a5494d475ea92e70c8b2287ff4 |
| Database: |
Directory of Open Access Journals |
