Therapeutic effects of sulforaphane in ulcerative colitis: effect on antioxidant activity, mitochondrial biogenesis and DNA polymerization

Bibliographic Details
Title: Therapeutic effects of sulforaphane in ulcerative colitis: effect on antioxidant activity, mitochondrial biogenesis and DNA polymerization
Authors: Abdullah Alattar, Reem Alshaman, Mohammed M. H. Al-Gayyar
Source: Redox Report, Vol 27, Iss 1, Pp 128-138 (2022)
Publisher Information: Taylor & Francis Group, 2022.
Publication Year: 2022
Collection: LCC:Pathology
LCC:Biology (General)
Subject Terms: Cyclin D1, heme oxygenase-1 (HO-1), mammalian target of rapamycin (mTOR), mitochondrial transcription factor A (TFAM), nuclear factor erythroid 2-related factor 2 (Nrf2), peroxisome proliferator-activated receptor-gamma coactivator (PGC-1), Pathology, RB1-214, Biology (General), QH301-705.5
More Details: Objectives Ulcerative colitis (UC), an inflammatory bowel disease, affects mucosal lining of colon leading to inflammation and ulcers. Sulforaphane is a natural compound obtained from cruciferous vegetables. We aimed to investigate potential therapeutic effects of sulforaphane in experimentally induced UC in rats through affection antioxidant activity, mitochondrial biogenesis and DNA polymerization.Methods UC was induced in rats via an intracolonic single administration of 2 ml of 4% acetic acid. UC rats were treated with 15 mg/kg sulforaphane. Samples of colon were used to investigate gene expression and protein levels of peroxisome proliferator-activated receptor-gamma coactivator (PGC-1), mitochondrial transcription factor A (TFAM), mammalian target of rapamycin (mTOR), cyclin D1, nuclear factor erythroid 2-related factor-2 (Nrf2), heme Oxygenase-1 (HO-1) and proliferating cell nuclear antigen (PCNA).Results UC showed dark distorted Goblet cell nucleus with disarranged mucus granules and no distinct brush border with atypical microvilli. All morphological changes were improved by treating with sulforaphane. Finally, treatment with sulforaphane significantly increased expression of PGC-1, TFAM, Nrf2 and HO-1 associated with reduction in expression of mTOR, cyclin D1 and PCNA.Conclusion Sulforaphane could cure UC in rats. The protective activity can be explained by enhancing antioxidant activity, elevating mitochondrial biogenesis and inhibiting DNA polymerization.
Document Type: article
File Description: electronic resource
Language: English
ISSN: 13510002
1743-2928
1351-0002
Relation: https://doaj.org/toc/1351-0002; https://doaj.org/toc/1743-2928
DOI: 10.1080/13510002.2022.2092378
Access URL: https://doaj.org/article/3d21c2d9de3f4cad8a098ce44cb756b4
Accession Number: edsdoj.3d21c2d9de3f4cad8a098ce44cb756b4
Database: Directory of Open Access Journals
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More Details
ISSN:13510002
17432928
DOI:10.1080/13510002.2022.2092378
Published in:Redox Report
Language:English