Bibliographic Details
| Title: |
Orientation-Controlled Electrocatalytic Efficiency of an Adsorbed Oxygen-Tolerant Hydrogenase. |
| Authors: |
Nina Heidary, Tillmann Utesch, Maximilian Zerball, Marius Horch, Diego Millo, Johannes Fritsch, Oliver Lenz, Regine von Klitzing, Peter Hildebrandt, Anna Fischer, Maria Andrea Mroginski, Ingo Zebger |
| Source: |
PLoS ONE, Vol 10, Iss 11, p e0143101 (2015) |
| Publisher Information: |
Public Library of Science (PLoS), 2015. |
| Publication Year: |
2015 |
| Collection: |
LCC:Medicine
LCC:Science |
| Subject Terms: |
Medicine, Science |
| More Details: |
Protein immobilization on electrodes is a key concept in exploiting enzymatic processes for bioelectronic devices. For optimum performance, an in-depth understanding of the enzyme-surface interactions is required. Here, we introduce an integral approach of experimental and theoretical methods that provides detailed insights into the adsorption of an oxygen-tolerant [NiFe] hydrogenase on a biocompatible gold electrode. Using atomic force microscopy, ellipsometry, surface-enhanced IR spectroscopy, and protein film voltammetry, we explore enzyme coverage, integrity, and activity, thereby probing both structure and catalytic H2 conversion of the enzyme. Electrocatalytic efficiencies can be correlated with the mode of protein adsorption on the electrode as estimated theoretically by molecular dynamics simulations. Our results reveal that pre-activation at low potentials results in increased current densities, which can be rationalized in terms of a potential-induced re-orientation of the immobilized enzyme. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1932-6203 |
| Relation: |
http://europepmc.org/articles/PMC4651547?pdf=render; https://doaj.org/toc/1932-6203 |
| DOI: |
10.1371/journal.pone.0143101 |
| Access URL: |
https://doaj.org/article/a3c00aadc1d04a1792fc41eb312d5d37 |
| Accession Number: |
edsdoj.3c00aadc1d04a1792fc41eb312d5d37 |
| Database: |
Directory of Open Access Journals |
