Bibliographic Details
| Title: |
Feasibility of Vascular Endothelial Growth Factor Imaging in Human Atherosclerotic Plaque Using Zr-Bevacizumab Positron Emission Tomography |
| Authors: |
Reza Golestani, Clark J. Zeebregts, Anton G.T. Terwisscha van Scheltinga, Marjolijn N. Lub-de Hooge, Gooitzen M. van Dam, Andor W.J.M. Glaudemans, Rudi A.J.O. Dierckx, René A. Tio, Albert J.H. Suurmeijer, Hendrikus H. Boersma, Wouter B. Nagengast, Riemer H.J.A. Slart |
| Source: |
Molecular Imaging, Vol 12 (2013) |
| Publisher Information: |
SAGE Publishing, 2013. |
| Publication Year: |
2013 |
| Collection: |
LCC:Biology (General)
LCC:Medical technology |
| Subject Terms: |
Biology (General), QH301-705.5, Medical technology, R855-855.5 |
| More Details: |
Intraplaque angiogenesis is associated with the occurrence of atherosclerotic plaque rupture. Cardiovascular molecular imaging can be used for the detection of rupture-prone plaques. Imaging with radiolabeled bevacizumab, a monoclonal anti-vascular endothelial growth factor (VEGF)-A, can depict VEGF levels corresponding to the angiogenic status in tumors. We determined the feasibility of 89 Zr-bevacizumab imaging for the detection of VEGF in carotid endarterectomy (CEA) specimens. Five CEA specimens were coincubated with 89 Zr-bevacizumab and aspecific 111 In-labeled IgG to determine the specificity of bevacizumab accumulation. In 11 CEA specimens, 89 Zr-bevacizumab micro-positron emission tomography (PET) was performed following 2 hours of incubation. Specimens were cut in 4 mm wide segments and were stained for VEGF and CD68. In each segment, the mean percent incubation dose per gram of tissue (%Inc/g) and tissue to background ratio were determined. A 10-fold higher accumulation of 89 Zr-bevacizumab compared to 111 In-IgG uptake was demonstrated by gamma counting. The mean %Inc/g hot spot was 2.2 ± 0.9 with a hot spot to background ratio of 3.6 ± 0.8. There was a significant correlation between the segmental tissue to background uptake ratio and the VEGF score (ρ = .74, p < .001). It is feasible to detect VEGF tissue concentration within CEA specimens using 89 Zr-bevacizumab PET. 89 Zr-bevacizumab accumulation in plaques is specific and correlates with immunohistochemistry scores. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1536-0121 |
| Relation: |
https://doaj.org/toc/1536-0121 |
| DOI: |
10.2310/7290.2012.00034 |
| Access URL: |
https://doaj.org/article/3a71e6d585634956b9cacf6b04c855eb |
| Accession Number: |
edsdoj.3a71e6d585634956b9cacf6b04c855eb |
| Database: |
Directory of Open Access Journals |
